Publication:
Strategic feeding of ammonium and metal ions for enhanced GLA-rich lipid accumulation in Cunninghamella bainieri 2a1

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dc.Chemicals/CASadenosine triphosphate citrate lyase, 9027-95-6; ammonia, 14798-03-9, 51847-23-5, 7664-41-7; calcium ion, 14127-61-8; cobalt, 7440-48-4; fatty acid synthase, 9045-77-6; ferric ion, 20074-52-6; glucose, 50-99-7, 84778-64-3; magnesium, 7439-95-4; malate dehydrogenase (decarboxylating), 9028-46-0, 9074-02-6, 9080-52-8; manganese, 16397-91-4, 7439-96-5; tartaric acid, 133-37-9, 3715-17-1, 526-83-0, 526-94-3, 87-69-4; zinc ion, 23713-49-7; Ammonium Compounds; Fatty Acids, Unsaturated; Metals
dc.citedby2
dc.contributor.affiliationsFaculty of Science and Technology
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorShuib S.
dc.contributor.authorWan Nawi W.N.N.
dc.contributor.authorTaha E.M.
dc.contributor.authorOmar O.
dc.contributor.authorAbdul Kader A.J.
dc.contributor.authorKalil M.S.
dc.contributor.authorAbdul Hamid A.
dc.date.accessioned2024-05-29T01:56:21Z
dc.date.available2024-05-29T01:56:21Z
dc.date.issued2014
dc.description.abstractStrategic feeding of ammonium and metal ions (Mg2+, Mn 2+, Fe3+, Cu2+, Ca2+, Co 2+, and Zn2+) for enhanced GLA-rich lipid accumulation in C. bainieri 2A1 was established. When cultivated in nitrogen-limited medium, the fungus produced up to 30% lipid (g/g biomass) with 12.9% (g/g lipid) GLA. However, the accumulation of lipid stopped at 48 hours of cultivation although glucose was abundant. This event occurred in parallel to the diminishing activity of malic enzyme (ME), fatty acid synthase (FAS), and ATP citrate lyase (ACL) as well as the depletion of metal ions in the medium. Reinstatement of the enzymes activities was achieved by feeding of ammonium tartrate, but no increment in the lipid content was observed. However, increment in lipid content from 32% to 50% (g/g biomass) with 13.2% GLA was achieved when simultaneous feeding of ammonium, glucose, and metal ions was carried out. This showed that the cessation of lipid accumulation was caused by diminishing activities of the enzymes as well as depletion of the metal ions in the medium. Therefore, strategic feeding of ammonium and metal ions successfully reinstated enzymes activities and enhanced GLA-rich lipid accumulation in C. bainieri 2A1. � 2014 Shuwahida Shuib et al.
dc.description.natureFinal
dc.identifier.ArtNo173574
dc.identifier.doi10.1155/2014/173574
dc.identifier.issn23566140
dc.identifier.pmid24991637
dc.identifier.scopus2-s2.0-84903555170
dc.identifier.scopusWOS:000337379500001
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84903555170&doi=10.1155%2f2014%2f173574&partnerID=40&md5=f9cb9594a70c05eb9dfcca78720421ae
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9826
dc.identifier.volume2014
dc.languageEnglish
dc.language.isoen_US
dc.publisherHindawi Publishing Corporation
dc.relation.ispartofOpen Access
dc.relation.ispartofScientific World Journal
dc.sourceScopus
dc.titleStrategic feeding of ammonium and metal ions for enhanced GLA-rich lipid accumulation in Cunninghamella bainieri 2a1
dc.title.alternativeSci. World J.
dc.typeArticle
dspace.entity.typePublication

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