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The use of Lates calcarifer as a biomarker for heavy metals detection

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dc.contributor.authorHayat, NMen_US
dc.contributor.authorShamaan, NAen_US
dc.contributor.authorSabullah, MKen_US
dc.contributor.authorShukor, MYen_US
dc.contributor.authorSyed, MAen_US
dc.contributor.authorKhalid, Aen_US
dc.contributor.authorDahalan, FAen_US
dc.contributor.authorAhmad, SAen_US
dc.date.accessioned2024-05-29T03:28:04Z
dc.date.available2024-05-29T03:28:04Z
dc.date.issued2016
dc.description.abstractFish are ubiquitous organisms that have many features that designate their potential as a biomarker of heavy metals pollution. Thus, an investigation was done to detect the effect of heavy metals on cholinesterase (ChE) activity from Lates calcarifer organs which were gill and muscle. Ammonium sulphate precipitation was performed along with ion exchange chromatography to purify the enzyme. In the substrate specificity study, ChE from L. calcarifer gills was capable of breaking down acetylthiocholine iodide (ATC) at a faster rate compared to the other two synthetic substrates, which are butyrylthiocholine iodide (BTC) and propionylthiocholine iodide (PTC). In contrast, the muscle ChE has a higher affinity towards PTC. The maximum activity of ChE observed at the temperature ranging from 20 to 30 A degrees C in Tris-HCl buffer pH 8. ChE from the two organs of L. calcarifer showed an inhibitive reaction towards heavy metals, but with different effects. ATC from gills showed 50 % inhibition by Cu, Hg and Pb, while PTC from muscle showed 50 % inhibition by Pb. The variation of inhibitory effect that was shown by ChE from L. calcarifer organs can be further studied in designing a biosensor kit that is sensitive towards heavy metal.
dc.identifier.doi10.1007/s12210-015-0501-7
dc.identifier.epage472
dc.identifier.isbn1720-0776
dc.identifier.issn2037-4631
dc.identifier.issue3
dc.identifier.scopusWOS:000381601300006
dc.identifier.spage463
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/12259
dc.identifier.volume27
dc.languageEnglish
dc.language.isoen_US
dc.publisherSpringer-Verlag Italia Srlen_US
dc.relation.ispartofRendiconti Lincei-Scienze Fisiche E Naturali
dc.sourceWeb Of Science (ISI)
dc.subjectBiomarkeren_US
dc.subjectBiosensoren_US
dc.subjectCholinesteraseen_US
dc.subjectHeavy metalsen_US
dc.titleThe use of Lates calcarifer as a biomarker for heavy metals detection
dc.typeArticleen_US
dspace.entity.typePublication

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