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The Role of Semipurified Fractions Isolated from Quercus infectoria on Bone Metabolism by Using hFOB 1.19 Human Fetal Osteoblast Cell Model

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dc.contributor.authorAmira Raudhah Abdullahen_US
dc.contributor.authorHermizi Hapidinen_US
dc.contributor.authorHasmah Abdullahen_US
dc.date.accessioned2024-05-28T03:52:07Z
dc.date.available2024-05-28T03:52:07Z
dc.date.issued2018
dc.description.abstractBackground. Quercus infectoria (QI) is a plant used in traditional medicines in Asia. The plant was reported to contain various active phytochemical compounds that have potential to stimulate bone formation. However, the precise mechanism of the stimulation effect of QI on osteoblast has not been elucidated. The present study was carried out to isolate QI semipurified fractions from aqueous QI extract and to delineate the molecular mechanism of QI semipurified fraction that enhanced bone formation by using hFOB1.19 human fetal osteoblast cell model. Methods. Isolation of QI semipurified fractions was established by means of column chromatography and thin layer chromatography. Established QI semipurified fractions were identified using Liquid Chromatography-Mass Spectrometry (LC-MS). Cells were treated with derived QI semipurified fractions and investigated for mineralization deposition and protein expression level of BMP-2, Runx2, and OPN by ELISA followed gene expression analysis of BMP-2 and Runx2 by RT-PCR. Results. Column chromatography isolation and purification yield Fractions A, B, and C. LC-MS analysis reveals the presence of polyphenols in each fraction. Results show that QI semipurified fractions increased the activity and upregulated the gene expression of BMP-2 and Runx2 at day 1, day 3, and day 7. OPN activity increased in cells treated with QI semipurified fractions at day 1 and day 3. Meanwhile, at day 7, expression of OPN decreased in activity. Furthermore, the study showed that combination of Fractions A, B, and C with osteoporotic drug (pamidronate) further increased the activity and upregulated the gene expression of BMP-2 and Runx2. Conclusions. These findings demonstrated that polyphenols from semipurified fractions of QI enhanced bone formation through expression of the investigated bone-related marker that is its potential role when combined with readily available osteoporotic drug.en_US
dc.identifier.citationAmira Raudhah Abdullah, Hermizi Hapidin, Hasmah Abdullah, "The Role of Semipurified Fractions Isolated from Quercus infectoria on Bone Metabolism by Using hFOB 1.19 Human Fetal Osteoblast Cell Model", Evidence-Based Complementary and Alternative Medicine, vol. 2018, Article ID 5319528, 13 pages, 2018. https://doi.org/10.1155/2018/5319528en_US
dc.identifier.doihttps://doi.org/10.1155/2018/5319528
dc.identifier.epage13
dc.identifier.issn1741-427X
dc.identifier.other2348-1
dc.identifier.spage1
dc.identifier.urihttps://www.hindawi.com/journals/ecam/2018/5319528/
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/5174
dc.identifier.volume2018
dc.language.isoenen_US
dc.publisherHindawien_US
dc.relation.ispartofEvidence-Based Complementary and Alternative Medicineen_US
dc.titleThe Role of Semipurified Fractions Isolated from Quercus infectoria on Bone Metabolism by Using hFOB 1.19 Human Fetal Osteoblast Cell Modelen_US
dc.typeArticleen_US
dspace.entity.typePublication

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