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Human mesenchymal stromal cells could deliver erythropoietin and migrate to the basal layer of hair shaft when subcutaneously implanted in a murine model

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dc.contributor.authorMok, PLen_US
dc.contributor.authorCheong, SKen_US
dc.contributor.authorLeong, CFen_US
dc.contributor.authorChua, KHen_US
dc.contributor.authorAinoon, Oen_US
dc.date.accessioned2024-05-29T02:50:53Z
dc.date.available2024-05-29T02:50:53Z
dc.date.issued2012
dc.description.abstractMesenchymal stromal cells (MSC) are an attractive cell-targeting vehicle for gene delivery. MIDGE (an acronym for Minimalistic, Immunologically Defined Gene Expression) construct is relatively safer than the viral or plasmid expression system as the detrimental eukaryotic and prokaryotic gene and sequences have been eliminated. The objective of this study was to test the ability of the human MSC (hMSC) to deliver the erythropoietin (EPO) gene in a nude mice model following nucleofection using a MIDGE construct. hMSC nucleofected with MIDGE encoding the EPO gene was injected subcutaneously in Matrigel at the dorsal flank of nude mice. Subcutaneous implantation of nucleofected hMSC resulted in increased hemoglobin level with presence of human EPO in the peripheral blood of the injected nude mice in the first two weeks post-implantation compared with the control groups. The basal layer of the hair shaft in the dermal layer was found to be significantly positive for immunohistochemical staining of a human EPO antibody. However, only a few basal layers of the hair shaft were found to be positively stained for CD105. In conclusion, hMSC harboring MIDGE-EPO could deliver and transiently express the EPO gene in the nude mice model. These cells could be localized to the hair follicle and secreted EPO protein might have possible role in hair regeneration. (C) 2012 Elsevier Ltd. All rights reserved.
dc.identifier.doi10.1016/j.tice.2012.04.002
dc.identifier.epage256
dc.identifier.issn0040-8166
dc.identifier.issue4
dc.identifier.scopusWOS:000321559500006
dc.identifier.spage249
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/11125
dc.identifier.volume44
dc.languageEnglish
dc.language.isoen_US
dc.publisherChurchill Livingstoneen_US
dc.relation.ispartofTissue & Cell
dc.sourceWeb Of Science (ISI)
dc.subjectMesenchymal stromal cellsen_US
dc.subjectNucleofectionen_US
dc.subjectMIDGEen_US
dc.subjectErythropoietinen_US
dc.subjectHemoglobinen_US
dc.subjectHair regenerationen_US
dc.titleHuman mesenchymal stromal cells could deliver erythropoietin and migrate to the basal layer of hair shaft when subcutaneously implanted in a murine model
dc.typeArticleen_US
dspace.entity.typePublication

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