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Influenza and respiratory syncytial viruses: Efficacy of different diagnostic assays

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dc.contributor.affiliationsFaculty of Medicine and Health Sciences
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.authorRahman M.M.en_US
dc.contributor.authorWong K.K.en_US
dc.contributor.authorAlfizah H.en_US
dc.contributor.authorHussin S.en_US
dc.contributor.authorIsahak I.en_US
dc.date.accessioned2024-05-28T08:26:17Z
dc.date.available2024-05-28T08:26:17Z
dc.date.issued2015
dc.description.abstractObjective: To determine the efficacy of cell culture, immunoflourescence Assay (IFA) and real time polymerase chain reaction (rRT-PCR) in relation to diagnosis of influenza and Respiratory Syncytial Virus (RSV). Methods: Total 2781 specimens of throat swabs and nasopharyngeal aspirates were obtained from patients suspected of respiratory viruses’ infections from January 2009 to December 2011 at Universiti Kebangsaan Malaysia Medical Centre(UKMMC).The specimens were processed by cell culture and immunoflurescence assay (IFA) and (rRT-PCR). Results: Thirty three (1.19%) specimens were positive for influenza virus A and 42 (1.51%) were positive for RSV by cell culture and IFA. On the other hand, rRT-PCR was able to identify 189 of 505 (37.43%) specimens in which 65 were influenza A virus and 124 were RSV. Sensitivity of rRT-PCR was 100% for both influenza A virus and RSV and specificity was 88% and 77% for influenza A virus and RSV, respectively. Conclusion: rRT-PCR diagnosed respiratory viruses in shorter time with a high level of sensitivity in comparison to conventional assays - cell culture and IFA. These advantages help in managing patients by saving cost and hospitalization stay.en_US
dc.description.natureFinalen_US
dc.identifier.CODENPJMSC
dc.identifier.doi10.12669/pjms.314.7003
dc.identifier.epage794
dc.identifier.issn1682024X
dc.identifier.issue4
dc.identifier.scopus2-s2.0-84937956099
dc.identifier.spage791
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84937956099&doi=10.12669%2fpjms.314.7003&partnerID=40&md5=c638ba63e4b8da5b30780ef5b59b86bf
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/8721
dc.identifier.volume31
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherProfessional Medical Publicationsen_US
dc.relation.ispartofOpen Accessen_US
dc.relation.ispartofPakistan Journal of Medical Sciences
dc.sourceScopus
dc.subjectCell cultureen_US
dc.subjectImmunoflurescence assayen_US
dc.subjectInfluenza virusen_US
dc.subjectReal-time polymerase chain reaction (rRT-PCR)en_US
dc.subjectRespiratory syncytial virusen_US
dc.titleInfluenza and respiratory syncytial viruses: Efficacy of different diagnostic assaysen_US
dc.title.alternativePak. J. Med. Sci.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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