Publication:
Establishment of Femoral Bone Defect Model in Sprague-Dawley Rat for Engineered Scaffold Implantation: A Pilot Study

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2024

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Springer

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Abstract

Animal models undeniably offer advantages for studying bone regeneration in bone tissue engineering. Currently, a lack of documented and standardized critical size defects (CSD) protocol exists for femoral bone. This study established a femoral bone rat model to evaluate engineered scaffold and its effect on bone regeneration. Eight Sprague-Dawley rats were divided into four groups, each induced with specific sizes of circular femoral defects: 1.5 mm diameter; 4.0 mm depth (Groups A and B), and 2.4 mm diameter; 7.0 mm depth (Groups C and D). Rats were euthanized at 4- and 8-weeks post-induction. Observations revealed that the 4-week period was insufficient for initiating the bone healing process. Notable signs of bone healing and remodelling become apparent only after 8 weeks with normal morbidity scoring at week 5 onwards. Gross examination indicated that rat models with a defect size of 1.5 mm diameter; and 4.0 mm depth healed at a faster rate suggesting inadequate defect size. In contrast, the rat model 2.4 mm diameter; and 7.0 mm depth defect emerged as the suitable model with evidence of newly formed bone signifying the process of mineralization at the defect site. The Hematoxylin and Eosin (H&E) staining of bone tissue demonstrates substantial formation of bone tissues (osteoid) and vascularized areas, consequently supporting the efficacy of this model. Therefore, this study finds that the 8-week timepoint with a 2.4 mm diameter and 7.0 mm circular defect is ideal for assessing bone regeneration of an engineered scaffold in rat bone model.

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9th European Medical and Biological Engineering Conference

Keywords

Bone regeneration · engineered scaffold · animal model · femoral bone defect · critical size defect

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https://oarep.usim.edu.my/handle/123456789/22362
 https://www.scopus.com/record/display.uri?eid=2-s2.0-85196055933&origin=resultslist&sort=plf-f&src=s&sid=06d2ac4b737633fd81e53d504fae4c3b&sot=b&sdt=b&s=TITLE-ABS-KEY%28Establishment+Of+Femoral+Bone+Defect+Model+In+Sprague-dawley+Rat+For+Engineered+Scaffold+Implantation%29&sl=116&sessionSearchId=06d2ac4b737633fd81e53d504fae4c3b&relpos=0
 https://link.springer.com/chapter/10.1007/978-3-031-61628-0_3

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