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Cytoprotective Role of Omentin Against Oxidative Stress-Induced Vascular Endothelial Cells Injury

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dc.contributor.authorKamaruddin, Nur Aqilah Bintien_US
dc.contributor.authorFong, Lai Yenen_US
dc.contributor.authorTan, Jun Jieen_US
dc.contributor.authorAbdullah, Muhammad Nazrul Hakimen_US
dc.contributor.authorCheema, Manraj Singhen_US
dc.contributor.authorBin Yakop, Fahmien_US
dc.contributor.authorYong, Yoke Keongen_US
dc.date.accessioned2024-05-29T03:26:02Z
dc.date.available2024-05-29T03:26:02Z
dc.date.issued2020
dc.description.abstractEndothelial cell injury caused by reactive oxygen species (ROS) plays a critical role in the pathogenesis of cardiovascular diseases. Omentin, an adipocytokine that is abundantly expressed in visceral fat tissue, has been reported to possess anti-inflammatory and antidiabetic properties. However, endothelial protective effects of omentin against oxidative stress remain unclear. This study aimed to evaluate the protective effect of omentin against hydrogen peroxide (H2O2)-induced cell injury in human umbilical vein endothelial cells (HUVECs). Cytotoxicity and cytoprotective effects of omentin were evaluated using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The apoptotic activity of HUVECs was detected using Annexin-V/PI and Hoechst 33258 staining methods. Antioxidant activity of omentin was evaluated by measuring both reactive oxygen species (ROS) levels and glutathione peroxidase (GPx) activity. No cytotoxicity effect was observed in HUVECs treated with omentin alone at concentrations of 150 to 450 ng/ml. MTT assay showed that omentin significantly prevented the cell death induced by H2O2 (p < 0.001). Hoechst staining and flow cytometry also revealed that omentin markedly prevented H2O2-induced apoptosis. Moreover, omentin not only significantly inhibited ROS production (p < 0.01) but also significantly (p < 0.01) increased GPx activity in HUVECs. In conclusion, our data suggest that omentin may protect HUVECs from injury induced by H2O2.en_US
dc.identifier.ArtNo2534
dc.identifier.doi10.3390/molecules25112534
dc.identifier.doihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85085909723&doi=10.3390%2fmolecules25112534&partnerID=40&md5=67d67eb7b963413ebc88005f9a6d1433
dc.identifier.issue11
dc.identifier.scopusWOS:000553858800071
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/12061
dc.identifier.volume25
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherMDPIen_US
dc.relation.ispartofMoleculesen_US
dc.subjectomentinen_US
dc.subjectoxidative stressen_US
dc.subjecthydrogen peroxideen_US
dc.subjecthuman umbilical veins endothelial cellen_US
dc.subjectendothelial injuryen_US
dc.titleCytoprotective Role of Omentin Against Oxidative Stress-Induced Vascular Endothelial Cells Injuryen_US
dc.typeArticleen_US
dspace.entity.typePublication

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