Options
Checklist of Marine Fungi and Yeasts Associated with Holothuria (Mertensiothuria) Leucospilota from Pangkor Island
Journal
Journal Of Sustainable Natural Resources
Date Issued
2020
Author(s)
Mohd Zulhafiz Che Zahri
Kamarul Rahim Kamarudin
Fatin Najihah Muhd Lutfi
Aisyah Mohamed Rehan
DOI
https://doi.org/10.30880/jsunr.2020.01.01.004
Abstract
Marine microorganisms such as fungi and yeasts can adapt to extreme marine environment conditions and play different roles especially in the nutrient cycling and as bioindicator of ocean change. This study was carried out to isolate and identify fungi and yeasts associated with Holothuria (Mertensiothuria) leucospilota from Pangkor Island, Perak, Malaysia in order to determine their species richness. Two specimens of H. leucospilota were collected from Giam Island and Teluk Nipah Beach of Pangkor Island. Nine samples of fungi and ten samples of yeasts were isolated from the internal and external parts of the H. leucospilota specimens such as cuticle, tentacle, coelomic fluid, cloaca, cuvierian tubules, and surrounding sediment and seawater. Polymerase Chain Reaction (PCR) and DNA sequencing of the Internal Transcribed Spacer (ITS) region were applied for species identification of the microorganisms. Sequence analyses of the ITS region resulted in the identification of five genera of fungi i.e. Cladosporium, Curvularia, Polyporaceae, Acremonium, and Penicillium; and four genera of yeasts i.e. Sterigmatomyces, Pichia, Debaryomyces, and Candida with some of them could be identified up to the species level. The findings have significantly contributed to the recent information on the checklist of fungi and yeasts isolated from the H. leucospilota specimens from Pangkor Island.
File(s)
Loading...
Name
Checklist of Marine Fungi and Yeasts Associated with Holothuria (Mertensiothuria) Leucospilota from Pangkor Island.pdf
Description
Checklist of Marine Fungi and Yeasts Associated with Holothuria (Mertensiothuria) Leucospilota from Pangkor Island
Size
346.8 KB
Format
Adobe PDF
Checksum
(MD5):17d8571ed892e50bbce94a619bf66b1f