Publication: Effects Of Cymbopogonflexuosus Essential Oil And Ph On Anti-coagulant Activity Of Extracellular Proteins Secreted By Bacillus Subtilis ATCC21332
Loading...
Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Department of Mathematics MC College
Abstract
Blood clotting is an emerging problem that seriously threatens the health of human
beings such as strokes and heart attacks. For the treatment, anti-coagulants are used in which
it could prevent and interrupt the process involved in the formation of blood clots. Microbial
proteases have now been attracted since it is cost effective and no side effects than typical
anti-coagulant agents. The protease enzymes were discovered from many microorganisms
including Bacillus sp. Previous study reported that B. subtilis could produce a serine protease,
known as Bacillopeptidase F with anti-coagulant activity. However, study on anti-coagulant
activity of microbial protein secreted by B. subtilis after being induced with
Cymbopogonflexuosus essential oil and cultured at difference pH media is still limited. In this
present study, B. subtilis ATCC21332 cells were treated with a low concentration (0.01 MIC)
of C. flexuosus essential oil and cultured in difference pH media (pH 6, pH 7 and pH 8) at
30°C for 72 h of fermentation. The extracellular proteins were then extracted and precipitated
by using 80% of ammonium sulfate before being further identified using Sodium Dodecyl
Sulfate-Polyacrylamide Gel Electrophoresis(SDS-PAGE) and tested for anti-coagulant
activity. SDS-PAGE analysis exhibited a proteins profile with a band in approximate size of
30 kDa was appeared for the treated bacteria with C. flexuosus essential oil and cultivated at
three difference pH of media. The extracellular proteins secreted by B. subtilis ATCC21332
after being treated with 0.01 MIC of C. flexuosus essential oil and cultivated either at pH 6 or
pH 7 or pH 8 could also prevent blood clotting. However, the extracellular proteins produced
by B. subtilis ATCC21332 without inducing with 0.01 MIC of C. flexuosus essential oil only
exhibited anti-coagulant activity when the bacterial cells were cultured at pH 7. As a
conclusion, B. subtilis ATCC21332 can be enhanced to produce anti-coagulant enzymes after
being treated with low concentration of C. flexuosus essential oil and cultivated in natural pH
media or even in slightly basic or slightly acidic environment. Further study should be done
to purify, identify and analyze the anti-coagulant enzymes from B. subtilis ATCC21332.
Keywords: Cymbopogonflexuosus Essential Oil, pH effect, Anti-Coagulants Activity,
Extracellular Proteins, Bacillus subtilis ATCC21332.
Description
Keywords
Cymbopogonflexuosus Essential Oil,, pH effect,, Anti-Coagulants Activity,, Extracellular Proteins,, Bacillus subtilis ATCC21332.
Citation
International Journal of Science, Environment and Technology, Vol. 6, No 5, 2017, 2944 – 2954