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https://oarep.usim.edu.my/jspui/handle/123456789/2226
Title: | In vitro and in vivo effects of Puntius javanicus cholinesterase by copper | Other Titles: | Fresenius Environ. Bull. | Authors: | Sabullah M.K. Sulaiman M.R. Shukor M.Y.A. Shamaan N.A. Khalid A. Ahmad S.A. |
Keywords: | Butyrylcholinesterase (BChE);Copper;P. javanicus | Issue Date: | 2015 | Publisher: | Parlar Scientific Publications | Journal: | Fresenius Environmental Bulletin | Abstract: | The aim of this study is to determine the inhibitory effect of copper towards butyrylcholinesterase (BChE) activity. Using the Lineweaver-Burk plot, Puntius javanicus BChE activity was found to be noncompetitively inhibited by copper. The maximal velocities of untreated (control) BChE, 0.5 and 10 mg/L copper-treated BChE are 53.70, 31.81 and 14.30 Umg-1, respectively, while the biomolecular constant (Km) values of both tests shows no significant difference (p>0.05). The in vitro IC50 of copper ion to the BChE was found to be 0.0948 (0.0658 to 0.1691) mg/L. In vivo tests showed that in the presence of 0.1 mg/L copper, the BChE activity was slightly higher compared to the untreated control. Copper sulfate at 0.3 mg/L concentration showed no significant inhibition compared to control. However, the activity decreased with increasing copper concentrations of 0.5, 1.0 and 5.0 mg/L, with the remaining activity at 87.60, 84.60 and 73.00 %, respectively. This study suggests that BChE isolated from P. javanicus liver tissue is a potentially new source of biomarker for copper contamination. � by PSP. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84958163599&partnerID=40&md5=86857a4c5c5cf5e37dfe3d87e898ca54 | ISSN: | 10184619 |
Appears in Collections: | Scopus |
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