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Title: | Biocatalytic production of lactose ester catalysed by mica-based immobilised lipase | Authors: | Zaidan, UH Rahman, MBA Othman, SS Basri, M Abdulmalek, E Rahman, RNZRA Salleh, AB |
Keywords: | Mica;Candida rugosa lipase;Covalent bonding;Cross-linking;Sugar esters | Issue Date: | 2012 | Publisher: | Elsevier Sci Ltd | Journal: | Food Chemistry | Abstract: | Enzyme immobilisation technology is an effective means to improve sugar ester production through the employment of biocatalysts. In the present study, immobilisation of Candida rugosa (CRL) lipase onto amino-activated mica is performed via covalent bonding (namely Amino-CRL) and the cross-linking of lipases into nano-reactors through physical adsorption (namely NER-CRL). Free and immobilised lipases were tested for their esterification activities. Specific activities for Amino-CRL and NER-CRL increased by 2.4 and 2.6-fold, respectively, upon immobilisation. Extending this work, immobilised lipases have novel capabilities in the synthesis of sugar esters. The optimised conditions for sugar fatty acid ester syntheses are 48 h at 2:1 of molar ratio of lactose sugar to capric acid at 55 C. Furthermore, a high operational stability with half-lives of over 13 and 10 runs was achieved for NER-CRL and Amino-CRL, respectively, indicating the efficiency of the immobilisation process. (C) 2011 Elsevier Ltd. All rights reserved. |
ISSN: | 0308-8146 | DOI: | 10.1016/j.foodchem.2011.08.060 |
Appears in Collections: | Web Of Science (ISI) |
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