106 
REFERENCES 
Abd El Gawad, I. A., A. M. Abd El Fatah & K. A. Al Rubbayyi. 2010. "Identification 
and characterization of dominant lactic acid bacteria isolated from traditional Rayeb 
milk in Egypt". Journal ofAmerican science. Vol. 6. (10): p. 728 -735. 
Abraham, A. G. & G. L Antoni. 1993. "Proteolytic activity of Lactobacillus bulgaricus 
grown in milk". Journal of Dairy Science. Vol. 76. p. 1488-1505. 
Ahmed, E. Y., C. Carolyn . 
2003. " Food Microbiology- A Laboratory Manual". John 
Wiley Sons, New Jersey. p. 153 -167. 
Ali, B. F., H. S. Chin & A. Raha. 2012. "Isolation and characterization of lactic acid 
bacteria from Polygonum minus. Romanian Biotechnological 17 (3): p. 7245- 7252. 
Akinkugbe, A. O. & A. A. Onilude. 2013. "Selective comparability and physiological 
studies of lactic acid bacteria protease and Calotropis procera (Linn) extracts". PeerJ 
PrePrints 1: e4vl. doi: 10.7287/peerj. preprints. 4. 
Andrews P. 1965. "The gel filtration behavior of proteins related to their molecular 
weights over a wide range". Biochemical Journal. Vol. 96. p. 595-605. 
Antoniou, K. D., D. Petridis, S. Raphaelides, Z. B. Omar, & R. Kesteloot. 2000. 
"Texture assessment of French cheeses". Journal Food Science. Vol. 65. p. 168-172. 
Anwer, M. A. Sarfraz, S. Aysha & A. Saeed. 2013. "Effect of Different Heating 
Temperature on Rhelolgical Properties of lactic Gel Made from Buffalo Milk". 
Journal of Food Chemistry and Nutrition. Vol. I (1):. p 33-41 
Argyle, G. M., & R. Chandan. 1976. "Production of cell wall bound proteinase by 
Lactobacillus bulgaricus and its location in bacterial cell. " Journal of Applied 
Bacteriology. Vol. 41. p. 175-184. 
Arima, K. Y. U. J., S. Iwasaki, & G. Tamura. 1968. "Milk-clotting enzyme from 
microorganisms: V. Purification and crystallization of Mucor rennin from Mucor 
pusillus var. lindt". Applied and environmental microbiology. Vol. 16. p. 172 -173. 
107 
Atamian, S. O. Ammar, K. B. Omar & T. Imad. 2014. "The characterization of the 
physicochemical and sensory properties of full-fat, reduced-fat and low-fat bovine, 
caprine, and ovine Greek yogurt (Labneh)". Food Science & Nutrition. Vol. 2. (2): p. 
164-173 
Aunstrup, K. 1980. " Proteinases". in: Economic Microbiology: Microbial Enzymes and 
Bioconversions, Vol. 5. p. 50-114., Academic Press. New York, 
Awad, S., . 2007. 
"Effect of sodium chloride and pH on the rennet coagulation and gel 
firmness". L WT- Food Science and Technology, Vol. 40. p. 220-224. 
Aween, M. M., Z. Hassan, B. J. Muhialdin, H. M. Noor &Y. A. Eljamel. 2012. 
"Evaluation on antibacterial activity of Lactobacillus acidophilus strains isolated from 
honey". American Journal Applied Science. Vol. 6. p. 807-817. 
Becker, T., & Z. Puhan. 1989. "Effect of Different Processes to Increase the Milk 
Solids Nonfat Content on the Rheological Properties of Yoghurt". Milchwissenschaft - 
Milk Science International. Vol. 44. p. 626-629. 
Belal J. M., Z. Hassan, M. M. A. Imdakim, Fredy K. S. A. & M. M. Aween. . 2012. "Malaysian Isolates of Lactic Acid Bacteria with Antibacterial Activity against Gram- 
Positive and Gram-Negative Pathogenic Bacteria". Journal of Food Research Vol. 1, 
p. 110-116. 
Bell, D. J, M. Hoare & P. Dunnill. 1983. "The formation of protein precipitates and 
their centrifugal recovery". Advance of Biochemistry and Engineering. Vol. 26 p. 1-72 
Ben Amor, K., E. Vaughan & W. de Vos. 2007. "Advanced molecular tools for the 
identification of lactic acid bacteria". Journal of Nutrition. Vol. 137. (3): p. 741. 
Benito, M., A. Martin, E. Aranda, F. Perez-Nevado, S. Ruiz-Moyano & M. Cordoba. 
2007. "Characterization and selection of autochthonous lactic acid bacteria isolated 
from traditional iberian dry-fermented salchichön and chorizo sausages". Journal of 
Food Science. Vol. 72. (6): p. 193-201. 
Beresford, T. P., N. A. Fitzsimons, N. L. Brennan, &, T. M. Cogan. 2001. "Recent 
advances in cheese microbiology" International Dairy Journal. Vol. 11. p. 259-274. 
108 
Bohdziewicz J. & M. Bodzek. 1994. "Ultrafiltration preparation of pectinolytic 
enzymes from citric acid fermentation broth, " Process Biochemistry. vol. 29, (2): p. 
99-107, 
Bradford, M. M. 1976. "A rapid and sensitive method for the quantitation of 
microgram quantities of protein utilising the principle of protein dye binding". 
Analytical Biochemistry. Vol. 72. p. 248-254. 
Bruno M. A., Cristian, M. L., Mana, E., Luara, M. L. L., Nestor, 0. &M. F. Pardo. 
2010. "Milk clotting and proteolytic activity of an enzyme preparation from Bromelia 
hieronymi fruits" LWT - Food Science and Technology. Vol. 43. p. 695-701 
Busch, U. & H. Nitschko. 1999. "Methods for the differentiation of microorganisms". 
Journal of chromatography B: Biomedical Sciences and Applications. Vol. 722 (2): 
P. 263-278 
Castillo, M., J. A. Lucey & F. A. Payne. 2006. "The effect of temperature and inoculum 
concentration on rheological and light scatter properties of milk coagulated by a 
combination of bacterial fermentation and chymosin Cottage cheese-type gels". 
International Dairy Journal. Vol. 16. p. 131-146. 
Cavalcanti, M. T. H., C. R. Martinez, V. C. Furtado, B. B. Neto, M. F. Teixera, F. J. L. 
Lima & A. L. F. Porto. 2005. "Milk-clotting protease production by Nocardiopsis sp. in 
an inexpensive medium". World Journal of Microbiology and Biotechnology. Vol. 
21. p. 151-154. 
Cerning, J., B. Christian, L. Michele & M. J. Desmazeaud. 1992. "Isolation and 
characterization of exopolysaccharides from slimeforming mesophilic lactic acid 
bacteria". Journal of Dairy Science. Vol. 75, p. 692-699. 
Chazarra, S, L. Sidrach, D. Lopez-Molina. & J. N. Rodriguez-Lopez. 2007. 
"Characterization of the milk-clotting properties of extracts from artichoke (Cynara 
scolymus, L. ) flowers". International Dairy Journal. Vol. 17. p. 1393-1400. 
Cheng, K., L. Fu-Ping, L. L. L. Ming, & L. Xiao-Mei. 2010. "Purification and 
biochemical characterization of a serine alkaline protease TC4 from a new isolated 
Bacillus alcalophilus TCCC 11004 in detergent formulations". African Journal of 
Biotechnology. Vol. 9. p. 4942-4953. 
109 
Cho, S. G. & H. H. Ki. 2006. "Isolation and Identification of Lactic Acid Bacteria 
Isolated from a Traditional Jeotgal Product in Korea" Ocean Science Journal. Vol. 41. 
. p. 113-119. 
Cho, W. H. . 
2007. "Optimization of extracellular alkaline protease production from 
species of Bacillus ". Journal of Industrial Microbiology Biotechnology. Vol. 34. p. 
241-245 
Choi, J. D., S. Home, & J. A. Lucey. 2007. "Effect of Insoluble Calcium 
Concentration on Rennet Coagulation Properties of Milk". Journal Dairy Science. 
Vol. 90. p. 2612-2623 
Christensen, C. M. 1984. "Food texture perception". Advances in Food Research, 
Vol. 29. p. 159-199. 
Chu, I. M., C. Lee, & T. S. Li. 1992. "Production and degradation of alkaline protease 
in batch cultures of Bacillus subtilis ATCC 14416". Enzyme and Microbial 
Technology. Vol. 14.755-761. 
Chye, S. J., R. Ahmad, & A. A Noor Aziah, 2012. "Studies on the physicochemical 
and sensory characteristics of goat's milk dadih incorporated with tropical- fruit 
purees" International Food Research Journal Vol. 19. p. 1387-1392. 
Cogan, T. M. 1996. History and taxonomy of starter cultures, In dairy starter cultures 
New 168 York (VCH Publishers) p. 1-20. Accolas (ed. ). Inc. 
Corredig, M. & D. G. Dalgleish. 1997. "Studies on the susceptibility of membrane- 
derived proteins to proteolysis as related to changes in their emulsifying properties". 
Food Research International Journal Vol. 30 p. 689 - 697. 
Couto, S. R. & M. A. Sanroman. 2006. "Application of solid state fermentation to food 
industry- A review", Journal of Food Engineering. Vol. 76. p. 291-302. 
Creamer L. K., A. Bienvenue, H. Nilsson, M. Paulsson, M. van Wanroij, E. K. Lowe, 
S. G. Anema, M. J. Boland, R. Jimenez-Flores. 2004. "Heat-induced redistribution of 
disulfide bonds in milk proteins. 1. Bovine (3-lactoglobulin". Journal of Agricultural 
and Food Chemistry. Vol. 25. p. 7660-7668. 
110 
Gutierrez-Mendez, N., N. Y., Trancoso-Reyes, M., Yarel, Y. Leal-Ramos. 2013. 
"Texture profile analysis of Fresh cheese and Chihuahua cheese using miniature 
cheese models" TECNOCIENCIA Chihuahua. Vol. 7. p. 65-74. 
Dalgleish, D. G . 
1992. "The enzymatic coagulation of milk". Advanced Dairy 
Chemistry. Vol. 1. p. 597-619 
Darah, I., M. L. Choo & S. H. Lim. 2013. "Influence of physical and chemical 
parameters on protease production by Bacillus megateruim IBRI MS 8.2 in submerged 
fermentation". Research Journal of Biological Sciences. Vol. 8. p. 54- 61 
Daviau, C., M. Famelart, H. G. E Pierre, & M. Jean- Louis. 2000. "Rennet Coagulation 
of Skim Alice Milk and Drainage: Effect of pH, Casein Concentration, ionic strength 
and heat treatment. INRA". Dairy Sciences and Thechnology Lait. Vol. 80. p. 397- 
415. 
De-Fernando, G. D. G, Hernandez, P. E, Burgos, 1., Sanz, B. & Ordohez, 1A.. 1991. " 
Extracellular proteinase from Enterococcusfaecalis subsp. liquefaciens. I Growth and 
extracellularproteinase production under different culture conditions". Folia 
Microbiol. Vol. 36, p 423-428. 
De-Lima, C. J. B., C. Mariana, R. B. Lovaglio, E. J. Ribeiro, J. Contiero, & E. H. De- 
Araüjo. 2008. "Production of rennet in submerged fermentation with the filamentous 
fungus Mucor miehei NRRL 3420". World Journal of Microbiology and 
Biotechnology. Vol. 4. p. 578-585. 
Dejmek, P., & P. Walstra. 2004. "The syneresis of rennet-coagulated curd". in Cheese: 
Chemistry, Physics and Microbiology Amsterdam, the Netherlands General Aspects 
Vol. 1. P. 70-103. 
De Vuyst, L. & F. Leroy. 2007. "Bacteriocins from lactic acid bacteria: production, 
purification, and food applications". Journal of Molecular Microbiology and 
Biotechnology. Vol. 13, p. 194-199. 
D'souza, T. M. & L. Pereira 1982. "Production and Immobilization of a Bacterial 
Milk-Clotting Enzyme". Journal of Dairy Science. Vol. 65. p. 2074-2081. 
ill 
Dutt, K., G. K. Meghwanshi, P. Gupta & R. K. Saxena. 2008. "Role of casein on 
induction and enhancement of production of a bacterial milk clotting protease from an 
indigenously isolated Bacillus subtilis ". Letters in Applied Microbiology. Vol. 46. p. 
513-518. 
Egitoa, A. S., J. M. Girardetc, L. E. Lagunaa, C. Poirsonc, B. L. Molle D'Micloc, G. 
Humbertc, & J. L. Gaillard 2007. "Milk-clotting activity of enzyme extracts from 
sunflower and albizia seeds and specific hydrolysis of bovine k-casein". International 
Dairy Journal. Vol. 17. p. 816-825. 
El-Bendary, M. A., M. E. Moharam & T. H. Ali. 2007. "Purification and 
characterization of milk-clotting enzyme produced by Bacillus sphaericus ". Journal 
ofApplied Sciences. Vol. 3. p. 695-699. 
El-Ghaish, S., I. Hadji-Sfaxi, A. Ahmadova, H. R. Choiset, M. Sitohy, T. Haertle & J. 
Chobert. 2011. "Characterization of two safe Enterococcus strains producing 
enterocins isolated from Egyptian dairy products" Beneficial Microbes Vol. 2, p. 17- 
25. 
El-Safey, E. M & U. M. Abdul-Raouf. 2004. "Production Purification and 
Characterization of Protease enzyme from Bacillus subtilis ". International 
Conferences for Development and the Environment in the Arab World. 14 
El-Sayed, S. T., M. M. A. Elmazar & R. A. Al-Azzouny. 2013. "Purification and 
Characterization of a Novel Milk Clotting Enzyme from Brassica napus Seeds". 
Australian Journal of Basic and Applied Sciences. Vol. 7. p. 482-493. 
E-Soda, M., N. Ahmed, N. Omran, G. Osman & A. Morsi. 2003. "Isolation, 
identification an selection of lactic acid bacteria cultures for cheese making". 
Emirates Journal of Food and Agriculture, Vol. 15. p. 51-71. 
Fadda, S., G. Vignolo, A. P. R. Holgado & G. Oliver. 1998. "Proteolytic Activity of 
Lactobacillus Strains Isolated from Dry fermented Sausages on Muscle 
Sarcoplasmic". Proteins Meat Science, Vol. 49. p. 11-18. 
Farzanfar, A. . 2006. 
"The use of probiotics in shrimp aquaculture" FEMS Immunology 
and Medical Microbiology, Vol. 48 p. 149-158. 
112 
Farrell, H. M., E. L. Malin, E. M. Brown & P. X. QI. 2006. "Casein micelle structure: 
What can be learned from milk synthesis and structural biology" Current Opinion in 
Colloid and Interface Science. Vol. 11. p. 135-147. 
Fernandez-Garcia, E., J. Tomillo & M. Nunez. 1999. "Effect of added proteinases and 
level of starter culture on the formation of biogenic amines in raw milk Manchego 
cheese". International Journal of Food Microbiology. Vol. 52, pp. 189-196. 
Fernandez, J., A. F. Mohedano, A. J. Polanco, M. Medina & M. Nunez. 1996. 
"Purification and characterization of an extracellular cystein proteinase produced by 
Micrococcus sp. INIA 528". Journal of Applied Bacteriology. Vol. 81. p. 27-34. 
Fernandez-Lahore, H. M., R. M. Auday, E. R. Fraile, 0. Cascone, M. Biscoglio de 
Jimenez Bonino, L. Pirpignani & C. Machalinski. 1999. "Purification and 
characterization of an acid proteinase from mesophilic Mucor sp. solid-state cultures". 
The Journal of Peptide Research. Vol. 53. p. 599-605. 
Fernandez P. A., F. M. Saguir & M. C. De Nadra. 2003. "Effect of amino acid and 
peptieds on growth of Pediococcus petosacceus from wine". Latin American Applied 
Research Vol. 33. P. 225-229. 
Fitzsimons, N. A., T. M. Cogan, S. Condon, & T. Beresford. 1999. "Phenotypic and 
genotypic characterisation of non-starter lactic acid bacteria in mature Cheddar 
cheese". Applied and Environmental Microbiology. Vol. 65, p. 3418-3426 
Foda M. S., E. M. Maysa, R. Amal & A. E. Magda. 2012. "Over production of milk 
clotting enzyme from Rhizomucor miehei through adjustment of growth under solid 
state fermentation conditions". Australian Journal of Basic and Applied Sciences, Vol. 
6. p. 579-589. 
Forde A. & G. F. Fitzgerald. 2000. "Biotechnological Approaches the Understanding 
and Improvement of Mature Cheese Flavour". Food Biotechnology., Vol. 11 p. 484- 
489. 
Fortina, G. M., P. Rossi, D. Mora, C. Parini, & E. Neviani. 1996. "Slow milk 
coagulating variants of Lactobacillus helveticus". Folia Microbiologica. Vol. 41. p. 33- 
38 
113 
Foucaud C., D. Hemme & M. Desmazeaud. 2008. "Peptide utilization by Lactococcus 
lactis and Leuconostoc mesenteroides ". Letters in Applied Microbiology. Vol. 32. p. 
20-25. 
Fox, P. F. 1989. "Proteolysis during cheese manufacture and ripening". Journal of 
Dairy Science. Vol. 72, p. 1379-1400. 
Fox, P. F. & Mc-Sweencey P. L. H. 1998. "Dairy chemistry and biochemistry 1 s" ad. 
London: Blackie Academic & professional 
Fox P. F. & L. Stepaniak. 1993. "Enzymes in cheese technology". International Dairy 
Journal. Vol. 3. P. 509-530. 
Ganesh, A. K., N. Nagesh, T. G. Prabhakan & G. Sekaran. 2008. "Purification of 
extracellular acid protease and analysis of fermentation metabilies by Synergistes sp. 
utilizing proteinaceous solid waste from tanneries. " Bioresource Technology Vol. 99 
p. 2364-2372. 
Giori, G. R., A. P. De Valdex, De Ruiz, Holgado, & G. Oliver. 1985. "Effects of pH, 
and temperature on the proteolytic activity of lactic acid bacteria". Journal of Dairy 
Science Vol. 68. p. 2160. 
Gobbettia, L. S., M. De Angelis, A. Corsetti, & R. Di Cagno, 2002 Latent bioactive 
peptides in milk proteins: proteolytic activation and significance in dairy processing. " 
Critical Reviews in Food Science and Nutrition. Vol. 42. p. 223-239. 
Goud, M. J. P., A. Suryam, V. Lakshmipathi & C. M. A Singara. 2009. "Extracellular 
hydrolytic enzyme profiles of certain South Indian basidiomycetes". African Journal 
of Biotechnology. Vol. 8. p. 354-360 
Guiama V. D., D. G. Libouga, E. Ngah, R. G. Beka, K. C. Ndi, B. Maloga, J. Bindzi, P. 
Donn & C. M. Mbofung. 2010 "Milk-clotting potential of fruit extracts from Solanum 
esculentum, Solanum macrocarpon L. and Solanum melongena". African Journal of 
Biotechnology. Vol. 9. P. 1797-1802. 
Guinee, T. P., Feeney, E. P., Auty, M. A. A. & RE Fox. 2002. "Effect of pH on calcium 
concentration on some textural and functional properties of Mozzarella cheese" Journa 
of Dairy Science. Vol. p. 1655-1669 
114 
Gunasekaran S. & A. k. A. Mehmet. 2003. "Cheese Rheology and Texture". CRC Press 
Boca Raton, Florida Ist ed. p. 377-397. 
Guyomarc, h. F., A. J. R. Law & D. G. Dalgleish. 2003. "Formation of soluble and 
micelle-bound protein aggregates in heated milk". Journal of Agricultural and Food 
Chemistry. Vol. 51. p. 4652-4660. 
Hajar, S. & T. H. T. A. Hamid. 2013. "Isolation of lactic acid bacteria strain 
Staphylococcus piscifermentans from Malaysian traditional fermented shrimp 
cincaluk". International Food Research Journal. Vol. 20. p. 125-129. 
Hajji, M. K.., S. Nasri., M. & N. Gharsallah. 2007. "Purification and characterization 
of an alkaline serine-protease produced by a new isolated Aspergillus clavatus ES 1 ". 
Process Biochemistry. Vol. 42 p. 791-797 
Handel, M. & E. R. Fraile. 1984. "Production of milk-clotting enzymes by aerated 
submerged culture of the strain of Mucor mucedo ". Acta Cientifzca Venezolana. 
Vol. 35 p. 111-115. 
Hansen C. L., A. S. Rinnan, S. B. Engelsen, T. Janhoj, E. Micklander, U. Andersen, 
F. & V. D. Berg. 2010. "Effect of Gel Firmness at Cutting Time, pH, and Temperature 
on Rennet Coagulation and Syneresis". Journal of. Agricultural and Food Chemistry. 
Vol. 58. p. 513-519 
Haque, Z. & J. E. Kinsella. 1988. "Interaction between heated x-casein and ß- 
lactoglobulin: predominance of hydrophobic interactions in the initial stages of 
complex formation". Journal of Dairy Research. Vol. 55. p. 67-80. 
Harwalkar V. R. & M. Kalab. 1986. "Relationship between Microstructure and 
Susceptibility to Syneresis in Yogurt Made from Reconstituted nonfat Dry Milk". 
Food Microstructure, Vol. 5. P. 287-294. 
He, X., F. Ren, H. Guo, W. Zhang, Song Xi & B. Gan. 2011. "Purification and 
properties of a milk-clotting enzyme producedby Bacillus amyloliquefaciens D4 ". 
Korean Journal of Chemical Engineering. Vol. 28. p. 203-208. 
115 
Hebert, E. M., G. S. De Giori & R. R. Raya. 2001. "Isolation and Characterization of a 
Slowly Milk-Coagulating Variant of Lactobacillus helveticus ". Applied and 
environmental microbiology. Vol. 67. p. 1846-1850. 
Haq-ul & H. Mukhtar. 2006. "Biosynthesis of protease from Lactobacillus paracasei : 
Kinetic analysis of fermentation parameters". Indian Journal of Biochemistry & 
Biophysics. Vol. 43. p. 377-381. 
Horikoshi, K. 1971. "Production of alkaline enzymes by alkalophilic microorganisms. 
Part I. Alkaline protease produced by Bacillus No. 221". Agriculture and Biological 
Chemistry. Vol. 35 p. 1407-1414 
Home, D. S. 2006. "Casein micelle structure: Models and muddles". Current Opinion 
in Colloid and Interface Science. Vol. 11. p. 148-153. 
Home S. . 1999. "Formation and structure of acidified milk gels". International Dairy 
journal. Vol. 9 p. 261 - 268. 
Hynes, E. R., C. A. Meinardy, N. Sabbag, T. Cattaneo, M. C. Candioti & C. A. Zalazar. 
2001. "Influence of milk-clotting enzyme concentration on the asl-casein hydrolysis 
during soft cheeses ripening". Journal of Dairy Science. Vol. 84 p. 1335-1340. 
Ibourahema, C. Dauphin, R. D. Jacqueline & P. Thonart. 2008. "Characterization of 
lactic acid bacteria isolated from poultry farms in Senegal, African Journal of 
Biotechnology. Vol. 7. p. 2006-2012. 
Irigoyen A., J. M. Izco, F. C. IbaAnÄez & P. Tone. 2001. "Influence of rennet milk- 
clotting activity on the proteolytic and sensory characteristics of an ovine cheese". 
Food Chemistry. Vol. 72. p. 137-144. 
Isma, A. M., M. Isao & E. B. Elfadil. 2009. "Characterisation of partially milk-clotting 
enzyme from Solanum dubium fresen seeds". Food Chemistry. Vol. 116: 395-400. 
Jaros, D., C. Heidig, & H. Rohm. 2007. "Enzymatic modification through microbial 
transglutaminase enhances the viscosity of stirred yogurt". Journal of Texture Studies, 
Vol. 38. p. 179-198. 
116 
Jaiswal P., R. Sharma, B. S. Sanodiya & P. S. Bisen. 2014. "Microbial 
Exopolysaccharides: Natural Modulators of Dairy Products". Journal of Applied 
Pharmaceutical Science. Vol. 4. p. 105-109. 
Joo, H. S., C. G. Kumar, G. C. Park, K. T. Kim, S. R. Paik & C. S. Chang. 2002. 
"Optimization of the production of an extracellular alkaline protease from Bacillus 
horikoshii ". Process Biochemistry. Vol. 38. p. 155- 159. 
Joo, H. S. & C. S. Chang 2005. "Production of protease from a new alkalophilic 
Bacillus sp. I-312 grown on soybean meal: optimization and some properties". 
Process Biochemistry. Vol. 40. p. 1263-1270. 
Juillard, V., H. Laan, E. R. S. Kunji, C. M. Jeronimus-Stratingh, A. P. Bruins & W. N. 
Konings. 1995. "The extracellular P 1-type proteinase of Lactococcus lactis hydrolyzes 
b-casein into more than one hundred different oligopeptides". Journal of Bacteriology, 
Vol. 177 p. 3472- 3478 
Khedid K., b, M. Faidc, A. Mokhtarib, A. Soulaymanib & A. Zinedined. 2009. 
"Characterization of lactic acid bacteria isolated from the one humped camel milk 
produced in Morocco" Microbiological Research. Vol. 164. p. 81-91. 
Karlsson, A. 0., R. Ipsen & Y. Ardo. 2007. "Influence of pH and NaCl on rheological 
properties of rennet-induced casein gels made from UF concentrated skim milk". 
International Dairy Journal. Vol. 17 p. 1053-1062. 
Kashket, S. & D. P. DePaola. 2002. "Cheese Consumption and the Development and 
Progression of Dental Caries". Nutrition Reviews. Vol. 60 p. 97 - 103. 
Kathiresan, K. & S. Manivannan. 2007. "Production of alkaline protease by 
Streptomyces sp. isolated from coastal mangrove sediment". Research Journal of 
Environmental Sciences. Vol. 1. p. 173-178. 
Kalisz, H. M., D. A. Wood & D. Moore. 1989. "Some characteristics of extracellular 
proteinases from Coprinus cinereus ". Mycological Research. Vol. 92. p. 278-285. 
Keila, A. M., T. H. Maria, S. D. Helena, B. T. Elias, H. M. Eduardo, A. L. Lins & P. 
Figueiredo. 2001. "Partial characterization of proteases from Streptomyce clavuligerus 
using an inexpensive medium". Brazilian Journal ofMicrbiology. Vol. 32. p. 76-82. 
117 
Khademi, F., S. Abachi, A. Mortazavi, M. A. Ehsani, M. R. Tabatabaei & F. A. 
Malekzadeh. 2013. "Optimization of fungal rennet production by local isolate of 
Rhizomucor nainitalensis under solid substrate fermentation system". Journal of 
Pharmacy and Biological Sciences. Vol. 5. p. 115-121. 
Kilcase, D. 1999. "Sensory techniques of study food texture". In food texture 
measurement & perception Gaitherburg, MD; Aspen Publishers Inc. (A. J. Rosenthal, 
ed) p. 30-64. 
Kim, W., K. Choi, Y. Kim, H. Park, J. Choi, Y. Lee, H. Oh, I. Kwon & S. Lee. 1996. 
"Purification and Characterization of a Fibrinolytic Enzyme Produced from Bacillus 
sp. strain CK11-4 Screened from Changkook-Jang" Applied and Environmental 
Microbiology. Vol. 62. p. 2482-2488. 
Koca, N. & M. Metin. 2004. "Textural, Melting and Sensory Properties of Low-fat 
Fresh Kashar Cheese Produced by Using Fat Replacers". International Dairy Journal. 
Vol. 14. p. 365-373. 
Kok, J. 1993. "Genetic and proteolytic enzymes of lactococci and their role in cheese 
flavor development". Journal of Dairy Sciences. Vol. 76. p. 2056-2064. 
Kumar, P. & H. N. Mishra. 2004. "Mango soy fortified set yoghurt: effect of stabilizer 
addition on physicochemical, sensory and textural properties". Food Chemistry. Vol. 
8. p. 501-507. 
Kunji, E. R. S., I. Mierau, A. Hagting, B. Poolman & W. N. Konings. 1996. "The 
proteolytic systems of lactic acid bacteria". Antonie van Leeuwenhoek. Vol. 70. p. 187- 
221. 
Kurutahalli, S., A. G. Vishwanatha, A. Rao & S. A. Singh. 2010. "Production and 
characterization of a milk-clotting enzyme from Aspergillus oryzae MTCC 5341". 
Applied Microbiology and Biotechnology. Vol. 85. p. 1849-1859. 
Laemmli UK . 1970. "Cleavage of structural proteins during the assembly of the head 
of bacteriophage T4". Nature Vol. 227. p. 680-685. 
Law, B. A. & J. Kolstad. 1983. "Proteolytic systems in lactic acid bacteria". Antonie 
Van Leeuwenhoek, Vol. 49. p. 225-245. 
118 
Lee, C. Y., M. F. Cheng, M. S. Yu & M. S. Pan. 2002. "Purification and characterization 
of a putative virulence factor, serine protease, from Vibrio parahaemlyticus". FEMS 
Microbiology Letters Vol. 209. p. 31-37. 
Lee, J. S., G. Y. Heo, J. W. Lee, Y. J. Oh, J. A. Park, Y. H. Park, Y. R. Pyun, & J. S. Ahn. 
2005. "Analysis of kimchi Microflora Using Denaturing Gradient Gel 
Electrophoresis". International Journal of Food Microbiology. Vol. Vol. 102. p. 143- 
150. 
Lee W. J. & J. A. Lucey. 2010. "Formation and Physical Properties of Yogurt". Asian 
Austarlasian Journal Animal Sciences. Vol. 23. p. 1127-1136. 
Leitner, G. 0. K., U. Merin, Y. Lavi & N. Silanikove. 2006. "Interactions between 
bacteria type, proteolysis of casein and physico-chemical properties of bovine milk". 
International Dairy Journal. Vol. 16. p. 648-654. 
Li Y., L. Shu, Z. Dejuan, C. Peng, S. Feng. & L. Hongyu. 2012. "Purification and 
characterization of Bacillus subtilis milk-clotting enzyme from Tibet Plateau and its 
potential use in yak dairy industry". European Food Research and Technology. Vol. 
p. 234: 733-741 
Llorente-Bousquets, A., M. Perez-Munguia & A. Farces. 2008. "Novel extracellular 
proteolytic acticity in Pediococcus acidilactici" ATTC 8042. Canadian Journal of 
Microbiology. Vol. 54. P. 694-699. 
Lucey, J. A. . 2002. 
"Formation and physical properties of milk protein gels". Journal 
of Dairy Science, Vol. 85. p. 281-294. 
Lucey, J. A. . 2001. "The Relationship between Rheological Parameters and 
Whey 
Separation in Milk gels". Food Hydrocolloids, Vol. 15. p. 603-608. 
Lucey, J. A., C. T. Teo, P. A. Munro & H. Singh. . 1998. "Microstructure, permeability 
and appearance of acid gels made from heated skim milk". Food Hydrocoids. Vol. 
p. 12: 159-165 
Lucey, J. A. . 2004. 
"Formation, structural properties and rheology of acid-coagulated 
milk gels". In Cheese: Chemistry, Physics and Microbiology. 3rd ed. Elsevier 
Academic Press, London. Vol. 1. p. 105-122 
119 
Ohhira, I. 1990. "Isolation and identification of lactic acid bacteria from traditional 
fermented sauce in Southeast Asia. " Japanese Journal of Dairy and Food Science, 
Vol. 39, p. 17 23. 
Olson, N. F. . 
1990. "The impact of lactic acid bacteria on cheese flavor". FEMS 
Microbiology Reviews Vol. 87, p131-147. 
Mahmood, W. & N. S. Hurmiz. 2012. "Improvement of Yogurt Properties by 
Microbial Transglutaminase". Jordan Journal ofAgricultural Sciences. Vol. 8. p. 333- 
342. 
Makarova, K., A. Slesarev, Y. Wolf, A. Sorokin, E. Koonin, A. Pavlov, N. Pavlova, 
V. Karamychev, N. Polouchin, V. Shakhova, I. Grigoriev, Y. Lou, D. Rohksar, S. 
Lucas, K. Huang, D. M. Goodstein, T. Hawkins, V. Plengvidhya, D. Welker, J. 
Hughes, Y. Goh, A. Benson, K. Baldwin, J. H. Lee, I. DiazMuniz, B. Dosti, V. 
Smeianov, W. Wechter, R. Barabote, G. Lorca, E. Altermann, R. Barrangou, B. 
Ganesan, Y. Xie, H. Rawsthorne, D. Tamir, C. Parker, F. Breidt, J. Broadbent, R. 
Hutkins, D. O'Sullivan, J. Steele, G. Unlu, Malbasa R. V., Vitas J. S., Loncar E. S., 
Kravi6. S. Z.. 2011. "Influence of Fermentation Temperature on the Content of Tatty 
Acids in Low Energy Milk-based kombucha Products. Acta Periodica Technologica, 
Vol. 42. p. 81-90. 
Malbasa, R. V., J. S. Vitas, E. S., Loncar S. Z. Kravis. 2011. "Influence of Fermentation 
Temperature on the Content of Tatty Acids in Low Energy Milk-based kombucha 
Products. Acta Periodica Technologica, Vol. 42. p. 81-90. 
Malik, R. K., R. Prasher & D. K. Mathur. 1994. "Genetics and production of 
extracellular polysaccharides by lactic acid bacteria -A review". Indian Journal of 
Dairy Science. Vol. 47. p. 977-984. 
Manolopoulou. E, P. Sarantinopoulos, E. Zoidou, A. Aktypis, E. Moschopoulou, I. G. 
Kandarakis & E. M. Anifantakis. 2003. "Evolution of microbial populations during 
traditional Feta cheese manufacture and ripening". International Journal. and Food 
Microbiol. Vol. 82. p. 153-161 
Mariela, A. B., C. M. Lazza, M. E. Errasti, L. M. I. Lopez, N. O. Caffini & M. F. Pardo. 
2010. "Milk clotting and proteolytic activity of an enzyme preparation from Bromelia 
hieronymi fruits". L WT - Food Science and Technology. Vol. 43. p. 695-701. 
120 
Mateo, M. J., D. J. O. Callaghan, C. D. Everard, C. C. Fagan, M. Castillo, F. A. Payne & 
C. P. O'Donnell. 2009. "Influence of Curd Cutting Programmed and Stirring Speed on 
the Prediction of Syneresis indices in Cheese-making using NIR Light Backscatter". 
L WT - Food Science and Technology. Vol. 42. P. 950-955. 
McEwan, J. A., D. Moore & J. S. Colwill. 1989. "The sensory characteristics of 
Cheddar cheese and their relationship with acceptability". Journal of the Society of 
Dairy Technology. Vol. 42. p. 112-117. 
McMahon, D. J., G. H. Richardson & R. J. Brown. 1984. "Enzymic milk coagulation: 
Role of equation involving coagulation time and curd firmness in describing 
coagulation". Journal of Dairy Science. Vol. 67. p. 1185-1193. 
McSweeney, P. L. H. & M. J. Sousa. 2000. "Biochemical pathways for the production 
of flavour compounds in cheese during ripening. A review". Lait. Vol. 80. p. 293-324. 
Mehrotra S, P. K. Pandey, R. Gaur & N. S. Darmwal. 1999. "The production of 
alkaline protease by a Bacillus species isolates". Bioresource Technology. Vol. 67. p. 
201-203. 
Melachouris, N. & S. L. Tuchey. 1987. "Properties of a Milk-Clotting Microbial 
Enzyme. Journal of Diary Sciences. Vol. 5 1. p. 650-655. 
Merheb, C. W., H. Cabral, E. Gomes, & R. Da-Silva. 2007. "Partial characterization 
ofprotease from thermophilic fungus Thermoascus aurantiacus and its hydrolytic 
activity on bovine casein". Food Chemistry. Vol. 104. p. 127-131. 
Merheb-Dini, C., E. Gomes, M. Boscolo & R. da Silva. 2010. "Production and 
characterization of a milk-clotting protease in the crude enzymatic extract from the 
newly isolated Thermomucor indicae-seudaticae N31: (Milk-clotting protease from 
the newly isolated Thermomucor indicae-seudaticae N31)". Food Chemistry Vol. 
120: 87-93. 
Mohd Adnan, A. F., & I. K. P. Tan. 2007. "Isolation of lactic acid bacteria from 
Malaysian foods and assessment of the isolates for industrial potential". Bioresource 
Technology. Vol. 98. p. 1380-1385. 
121 
Moon, S. H. & S. J. Parulekar. 1991. "A parametric study of protease production in 
batch and fedbatch cultures of Bacillus frrmus ". Biotechnology and Bioengineering. 
Vol. 37. p. 467-483. 
Moosavi-Nasab, M., M. Radi & H. A. Jouybari. 2010. "Investigation of enzyme 
modified cheese production by two species of Aspergillus". African Journal of 
Biotechnology. Vol. 9. p. 508-511. 
Mostafa, E. E., M. S. Moataza, Mohsen, H. S&D. E. El-Hadedy. 2013. "Isolation of 
clt Genes Encoding milk clotting enzyme and characterization of milk clotting enzyme 
from new isolate Streptomyces pseudogriseolus NRC-15" American Journal of 
Biotechnology Molecular Sciences. Vol. 3. p. 8-23. 
Muller-Buschbaum, P. M. u. R. Gebhardt, S. V. Roth., E. Metwalli & W. Doster. 2007. 
"Effect of Calcium Concentration on the Structure of Casein Micelles in Thin Films". 
Biophysical Journal. Vol. 93. p. 960-968. 
Nadra M. C. M. . 
2007. "Nitrogen Metabolism in Lactic Acid Bacteria from Fruits: a 
review". www. formatex. org/microbio/pdf/Pages500-510. pdf. 
Nanasombat, S, S. Phunpruch & T. Jaichalad. 2012. "Screening and identification of 
lactic acid bacteria from raw seafoods and Thai fermented seafood products for their 
potential use as starter cultures". Songklanakarin Journal of Science and Technology. 
Vol. 34 p. 255-262. 
Naidu, K. S. B. & K. L. Devi. 2005. "Optimization of thermostable alkaline protease 
production from species of Bacillus using rice bran". African Journal of 
Biotechnology 4: 724-726. 
Nagodawithana, T. & G. Reed. 1993. Enzymes in Food Processing. 3rd Edn. San 
Diego, USA: Academic Press. 
Nateghi, L., S. Roohinejad, A. Totosaus, H. Mirhosseini, M. Shuhaimi, A. 
Meimandipour, A. Omidizadeh, & M. Y. Abd-Manap. 2012. "Optimization of 
Textural Properties and Formulation of Reduced Fat Cheddar Cheeses Containing Fat 
Replacers". Journal of Food, Agiculture and Environment. Vol. 10. p. 46-54. 
122 
Ng-Kwai-Hang, K. F. 
. 2002. "Milk Proteins Heterogeneity, Fractionation and Isolation". Encyclopedia of Dairy Sciences p. 1881-1894 
Nigam, J. N., K. R. Pillai & J. N. Baruah. 1981. "Effect of carbon and nitrogen sources 
on neutral proteinase production by Pseudomonas aeruginosa". Folia Microbiology. 
Vol. 26. p. 358-363. 
Najera A. J., M. Renobales & L. J. R. Barron. 2003. "Effect of pH, temperature, CaC12 
and enzyme concentrations on the rennet-clotting properties of milk. A multifactorial 
study". Food Chemistry. Vol. 80, p. 345-352. 
Nikita C. & D. Hemangi. 2012. "Isolation, Identfication and Characterization of 
Lactic acid bacteria from Dairy Slude Sample". Journal of Environmental Research 
And Development. Vol. 7. p. 234-244. 
Noomen, A. 1978. "Activity of proteolytic enzymes in simulated soft cheeses 
(Meschanger type) 1. Activity of milk protease". Netherlands Milk and Dairy Journal. 
Vol. 32, p. 26-49. 
Nouani, A., N. Belhamice, R. Slamani, S. Belbraouet, F. Fazouane & M. M. Bellal. 
2009. "Extracellular protease from Mucor pusillus: purification and characterization". 
International Journal of Dairy Technology. Vol. 62. p. 112-117. 
Nour I., F.. Faiza &E Hala. 2014. "Chemically Defined Medium for Optimization of 
Proteolytic Activity of Lactobacillus bulgaricus 761N". International Journal of Life 
Science and Medical Research. Vol. 4. p. 46-56. 
Ohhira, I. 1990. "Isolation and identification of lactic acid bacteria from traditional 
fermented sauce in Southeast Asia". Japanese Journal of Dairy and Food Science. 
Vol. 39. p. 17-23. 
Oke, M. A. & M. B. Odebisi. 2014. "Partial Purification and Characterization of 
Extracellular Protease from Pedicoccus acidilactici ". Nigerian Journal of Basic and 
Applied Science. Vol. 22. p. 19-25. 
Olaoye, O. A. & A. A. Onilude, 2008. "Identification of pediococcus ssp. from beef 
and evaluation of their lactic acid production in varying concentrations of different 
carbon sources. Advances in natural and applied sciences, Vol. 2. p. 197-207. 
123 
Olson, N. F. 1990. "The impact of lactic acid bacteria on cheese flavor". FEMS 
Microbiology Reviews. Vol. 87. p. 131-147. 
Osuntoki, A. & Korie, I.. 2010. "Antioxidant activity of whey from milk fermented 
with Lactobacillus species isolated from Nigerian fermented foods". Food Technology 
and Biotechnology. Vol . 48. p. 505-511. 
Oyewole O. A. & P. Isah. 2012. "Locally Fermented Foods in Nigeria and their 
Significance to National Economy: a Review". Journal of Recent Advances in 
Agriculture. Vol. 4. p. 92-102. 
Pailin, T., D., Kang,, K., Schmidt, & Fung, D. 2001. Detection of extracellular bound 
proteinase in EPS-producing lactic acid bacteria cultures on skim milk agar. Letters in 
Applied Microbiology, Vol. 33, pp. 45-49. 
Papamanoli, E., N. Tzanetakis, E. Litopoulou-Tzanetaki & P. Kotzekidou. 2003. 
"Characterization of lactic acid bacteria isolated from a Greek dry-fermented sausage 
in respect of their technological and probiotic properties". Meat Science. Vol. 65. p. 
859-867. 
Patel, R., M. Dodia & S. P. Singh. 2005. "Extracellular alkaline protease from a newly 
isolated haloalkaliphilic Bacillus sp.: Production and optimization". Process 
Biochemistry. Vol. 40. p. 3569-3575. 
Pei-Jing, Y. & C. C, Chou . 2005. "Factors Affecting the 
Growth and Production of 
Milk Clotting Enzyme by Amylomyces rouxii in Rice Liquid Medium". Food 
Technology and. Biotechnology Vol.. 43 p. 283-288 
Peter L. B., W. M. Hugh & D. Saul. 2014 "Selected Enzymes from Extreme 
Thermophiles with . 
Applications in Biotechnology. " Current Biotechnology. 
Vol. 3 p. 45-59. 
Pezeshki A., J. Hesari I, A. A. Zonoz, & B. Ghambarzadeh. 2011. "Influence of 
Withania coagulans Protease as a Vegetable Rennet on Proteolysis of Iranian UF 
White Cheese" Journal ofAgricultural Science and Technology. Vol. 13. P. 567-576. 
Phadungath, C. 2005. "Casein micelle structure: a concise: A review". Songklanakarin 
Journal of Science and Technology, Vol. 27 p. 201-212. 
124 
Pires M. S., G. A. Orellana & C. A. Gatti. 1999. "Rennet coagulation of casein micelles 
and heated casein micelles: action of Ca2+ and pH. " Food Hydrocolloids. Vol. 13. p. 
235-238. 
Porto A. L. F., G. M., Campos-Takaki & J. L. Lima- Filho. 1996. "Effect of culture 
condation on protease production by Streptomyces clavuligerus growing soybean flour 
medium. " Applied Biochemistry and Biotechnology, Vol. 60. p. 115- 122. 
Poza, M, C. Sieiro, L. Carreira, J. Barros-Velazquez & T. G. Villa. 2003. "Production 
and characterization of the milk-clotting protease of Myxococcus xanthus strain 422". 
Journal of Industrial Microbiology & Biotechnology. Vol. 30. p. 691-698. 
Pritchard, G. G. & T. Coolbear. 1993. "The Physiology and Biochemistry of the 
Proteolytic System in Lactic Acid Bacteria: Review". FEMS Microbiology. Vol. 12. 
p. 179-206. 
Puri, S., Q. Khalil-Beg & R. Gupta. 2002. "Optimization of Alkaline Protease 
Production from Bacillus sp. by Response Surface Methodology". Current 
Microbiology. Vol. 44. p. 286-290. 
Rao, L. K. & D. K. Mathur. 1979. "Assessment of purified bacterial milk clotting 
enzyme from bacillus subtilis K-26 for cheddar cheese making". Journal of Dairy 
Scencei. Vol. 62. p. 378-383. 
Rashidi, H., M. Mazaheri-Tehran, S. M. A. Razavi, & M. Ghods-Rohany. 2015. 
"Improving Textural and Sensory Characteristics of Low-Fat UF Feta Cheese Made 
with Fat Replacers". Journal of Agriculture Science and Technology. Vol. 17. p. 121- 
132. 
Reps, A., L. Jedrychowski, K. Wisniewska, & A. Jankowska. 2006. "Application of a 
coagulating preparation obtained with Rhizomucor miehei N in cheese-making". 
Pakistan Journal of Nutrition. Vol. 5. p. 97-101. 
Richardson, G. H., J. H. Nelson & R. E. Lubnow. 1967. "Rennin like enzyme from 
Mucor pusillus for cheese manufacture". Journal of Dairy Sciences. Vol. 50. p. 1066- 
1072. 
125 
Rifaat, H. M., S. M. Hassanein, O. H. El-Said, S. A. Saleh & M. S. M. Selim. 2006. 
"Purification and characterisation of extracellular neutral protease from Streptomyces 
microflavus". Arab Journal of Biotechnology. Vol. 9. p. 51-60575. 
Rita, C. S. N. S., Valeria P. R. M., Marcia C. R. T. V., Alexandre N. S., Andrea A. S. 
& A. M. Luis. 2012. "Sensory and Instrumental Consistency of Processed Cheeses" 
Journal of Food Research. Vol. 1. p. 204-342 
Rhee, S. J., J. Lee & Lee Ch. 2011 "Importance of lactic acid bacteria in Asian 
fermented foods". Microbial Cell Factories. p. 55. 
Roseiro, L. B., M. Barbosa, J. M. Ames, & A. Wilbey. 2003. "Cheesemaking with 
vegetable coagulants-The use of Cynara L. for the production of ovine milk cheese". 
International Journal of Dairy Technology. Vol. 56. p. 76-85. 
Rudan, M. A., D. M. Barbano, M. R. Guo & P. S. Kindstedt. 1998. "Effect of die 
modification of fat particle size by homogenization on composition, proteolysis, 
fimctionality and appearance of reduced fat mozzarella cheese". Journal of Dairy 
Science. Vol. 81. p. 2065-2076. 
Saier, M. K., T. P. Richardson, S. Kozyavkin, B. Weimer & D. Mills. 2006. 
"Comparative genomics of the lactic acid bacteria". Proceedings of the National 
Academy of Sciences. USA. Vol. 103. p. 15611-15616. 
Samson, A., S. Chen & J. Zhao. 2004. "Formation of bitter peptides during ripening of 
ovine milk cheese made with different coagulants". Lait EDP Sciences. Vol. 84 p. 
567-578. 
Sandra, S, M. Ho, M. Alexander & M. Corredig. 2012. "Effect of soluble calcium on 
the renneting properties of casein micelles as measured by rheology and diffusing 
wave spectroscopy". Journal of Dairy Sciences. Vol. 95. p. 75-82. 
Sannabhadti, S. S. & R. A. Srinivasan. 1977. "Milk clotting enzymes from Abisidia 
ramosa. Part 1. Factors influencing production". Indian Journal of Dairy Science. Vol. 
30. p. 331-335 
126 
Saranraj, P., M. A. Naidu & P. Sivasakthivelan. 2013. "Lactic Acid Bacteria and its 
Antimicrobial Properties". International Journal of Pharmaceutical & Biological 
Archives. Vol. 4. p. 1124 - 113.. 
Sathya, R., S. P. Francis, A. Jayaraman, & M. Palaniswamy. 2013. ` Influence of 
microbial rennet on the production of cheddar cheese madeusing cow milk" Asian 
Pacific Journal of Tropical Biomedicine, Vol. p. 1-7. 
Sato, S., H. Tokuda, T. Koizumi & K. Nakanishi. 2004. "Purification and 
Characterization of an Extracellular Proteinase Having Milk-Clotting Activity from 
Enterococcusfaecalis TUA2495L". Food Science and Technology Research. Vol. 10. 
p. 44-50. 
Savijoki, K., H. Ingmer & P. Varmanen. 2006. "Proteolytic systems of lactic acid 
bacteria". Applied Microbiology and Biotechnology. Vol. 71. p. 394-406. 
Sbodio, 0. A. E. J. Tercero, R. Coutaz & G. R Revelli. 2006. "Effect of Rennet and 
Sodium Chloride Concentration on Milk Coagulation Properties". Ciencia e 
Tecnologia de Alimentos. Vol. 5. p. 182-188. 
Secades, P. & J. A. Guijarro. 1999. "Purification and characterization of an 
extracellular protease from the fish pathogen Yersinia ruckeri and effect of culture 
conditions on production". Applied and Environmental Microbiology, Vol. 65. p. 
3969-3975. 
Serra, M., A. J. Trujillo, J. M. Quevedo, B. Guamis & V. Ferragut. 2007. "Acid 
Coagulation Properties and Suitability for Yogurt Production of Cow's Milk Treated 
by High-pressure Homogenisation". International Dairy Journal, Vol. 17. p. 782-790. 
Shata, H. M. A. 2005. "Extraction of milk-clotting enzyme produced by solid state 
fermentation of Aspergillus oryzae ". Poilish Journal of Microbiology, Vol. 54. p. 241- 
247. 
Shieh, C. J., L. A Phan-Thi & I. L. Shih. 2009. "Milk clotting enzymes produced by 
culture of Bacillus subtilis natto". Biochemical Engineering Journal, Vol. 43. p. 85-91. 
127 
Shivanand, P. & G. Jayaraman, 2009. "Production of extracellular protease from 
halotolerant bacterium, Bacillus aquimaris strain VITP4 isolated from Kumta coast". 
Process. Biochemistry Vol. 10. p. 1088-1094. 
Shumi, W., H. N M. D. Towhid & M. N. Anwar. 2004. "Production of Protease from 
Listeria monocytogenes " International journal of Agriculture and Biology. Vol. 6. 
p. 1097-1100. 
Silveira, G. G., G. M. Oliveira, E. J. Ribeiro, R. Monti, & J. Contiero. 2005. "Microbial 
rennet produced by Mucor miehei in solid state and submerged fermentation". 
Brazilian Archives of Biology and Technology, Vol. 6. p. 931-937. 
Sim, K. Y., F. Y. Chye & A. Anton. 2009. "Microbiological characterization an 
indigenous Malaysian fish sauce. " Borneo science. Vol. 24 p. 25-35. 
Sipahioglu, 0., V. B. Alvarez, & C. Solano Lopez. 1999. "Structure, Physicochemical 
& Sensory Properties of Feta Cheese Made with Tapioca Starch and Lecithin as Fat 
Mimetic, International. Dairy Journal, Vol. 9 p. 783-789. 
Sodini, I., J. Mattas & P. S. Tong. 2006. "Influence of pH and Heat Treatment of 
Whey on the Functional Properties of Whey Protein Concentrates in Yoghurt". 
International Dairy Journal. Vol. 16. p. 1464-1469. 
Soledad, C., L. Sidrach, D. Lopez-Molin & J. N. Rodnguez-Lopez. 2007. 
"Characterization of the milk clotting properties of extracts from Artichoke (Cynara 
scolymus, L. ) flowers". International Dairy of Journal. Vol. 17. p. 1393-1400. 
Sousa, M. J., Y. Ardo & P. L. H. McSweeney. 2001. "Advances in the study of 
proteolysis in cheese during ripening". International Dairy Journal. Vol. 11. p. 327- 
345. 
Srinivasan, M. & S. C. Dhar. 1990. "Factors influencing extracellular synthesis in an 
Aspergillus flavus isolate". Acta Microbiologica et Immunologica Hungarica. Vol. 37. 
p. 15-23. 
Smit, G., A. Verheul, R. van Kranenburg, E. Ayad, R. Siezen, & W. Engels. 2002. 
"Cheese flavour development by enzymatic conversions of peptides and amino acids". 
Food Research International. Vol. 33. p. 153-160(8). 
128 
Stoeva, St. P. & B. K. Mesrob, 1977. "Proteolytic and Esterolytic Activity of a Milk 
Clotting Protease (MCP) from Bacillus mesentericus strain 76" FEBS Letter. Vol. 
Vol. 80. p. 86-88. 
Surmacka-Szczesniak, A. 
. 
2002. "Texture is a sensory property". Food Quality and 
Preference, Vol. 13. p. 215-225. 
Swearingen, P. A., D. J. O'Sullivan & J. J. Warthesen, 2001 "Isolation, characterisation, 
and influence of native, non-starter lactic acid bacteria on Cheddar cheese quality". 
Journal of Dairy Science. Vol. 84, p. 50-59. 
Szczesniak, A. S. 1963. " Classification of textural characteristics". Journal of Food 
Science, Vol. 28. p. 385-389. 
Tamime, A. Y. & R. K. Robinson. 1999. "Yoghurt: Science and Technology". CRC 
Press, Boca Raton, 2nd ed. FLorida. 
Tanasuoawat, S. & Komagata. 1995. "Lactic acid bacteria in fermented foods in 
Thailan" World Juornal of microbiology and Biotechnology. Vol. 11 . p. 
253- 256. 
Tang, Y. W., N. M. Ellis, M. K. Hopkins, D. H. Smith, D. E. Dodge & D. H. Persing. 
1998. "Comparison of phenotypic and genotypic techniques for identification of 
unusual aerobic pathogenic gram-negative bacilli". Journal of Clinical Microbiology, 
Vol. 36. p. 3674-3679. 
Temmerman, R., G. Huys & J. Swings. 2004. "Identification of Lactic Acid Bacteria: 
Culture-Dependent and Culture-Independent Methods". Trends Food Science and 
Technology. Vol. 15. p. 348-359. 
Thomast, . D. & E. Millso. 1981. 
"Proteolytic enzymes of starter bacteria". 
Netherlands Milk and Dairy Journal Vol. 35. p. 255-273. 
Tsujibo, H. K. M., T. Hasegawa & Y. Inamori. 1990. "Purification and 
characterization of two types of alkaline serine proteases produced by an alkaliphilic 
actinomycete. " Journal ofApplied. Bacteriology. Vol. 69 p. 520-529. 
129 
Tunick, M. H., E. L. Malin, P. W. Smith, J. J. Shieh, B. C Sullivan, K. L. Mackey, & 
V. H. Holsinger. 1993. "Proteolysis and rheology of low fat and fill fat mozzarella 
cheeses prepared from homogenised milk". Journal of Dairy Science. Vol. 76. p. 3621- 
3628. 
Tunga, R., B. S. & R. Banerjee. 2003. "Purification and characterization of a protease 
from solid state cultures of A. parasiticus ". Process Biochemistry, Vol. 32. p. 1553- 
1558. 
Udomsil, N., S. S. T. Rodtong, & J. Yongsawatdigul. 2010. "Proteinase-producing 
halophilic lactic acid bacteria isolated fromfish sauce fermentation and their ability to 
produce volatile compounds, International" Journal of Food Microbiology Vol. 141. 
p. 186-194. 
Uyar F., Ilknur P., Goksel K., Yilmaz 
. 
2011. "Optimal conditions for production of 
extracellular protease from newly isolated Bacillus cereus strain CAI 5 European Asia 
Journal Biology Science, Vol. 5. p. 1-9. 
Van Vliet T., C. M. M., Lakemond, & R. W. Visschers. 2004. "Rheology and structure 
of milk protein gels". Current Opinion in Colloid & Interface Science Vol. 9. p. 298- 
304. 
Vairo-Cavalli, S., S. Claver, N. Priolo & C. Natalucci. . 2005. 
"Extraction and partial 
characterization of a coagulant preparation from Silybum marianum flowers. Its action 
on bovine caseinate. " Journal of Dairy Research. Vol. 72. p. 271-275. 
Verma, A. K., V. P. Singh & V. Pathak, 2012. "Effect of calcium chloride salton the 
curding of cross breed coe milk- a case report. " Asian Journal of Science and 
Technology Vol. 4. p. 28-31. 
Visser, S. 1993. "Proteolytic enzymes and their relation to cheese ripening and 
flavour: an overview". Journal of Dairy Research Vol. 76. p. 329-350. 
Vishwanatha, K. S., A. A. G. Rao & S. A. Singh. 2010. "Production and characterization 
of a milk-clotting enzyme from Aspergillus oryzae MTCC 5341". Applied 
Microbiology and Biotechnology, Vol. 85. p. 1849-1859. 
130 
Vladimir R. V., G. K., Katarina D. M., Spasenija, D. I., Mirela, V. H., Dajana & I. R. 
Marjan 
. 
2014. "Correlation of the microstructure with viscosity Texture properties 
during milk fermentation by Kombucha inoculum". BIBLID. Vol. 45. p. 89- 98. 
Wium, H., K. R., Kristiansen & K. B. Qvist. 1998. "Proteolysis and Its Role in Relation 
to Texture of Feta Cheese Made from Ultrafiltered Milk with Different Amounts of 
Rennet". Journal of Dairy Research. Vol. 65. p. 665-674 
Walstra, P. 1993. "The syneresis of curd in Cheese: chemistry, physics and 
microbiology". Chapman & Hall, London. Second ed). Vol. 1. p. 141-191. 
Walstra, P., T. J. Geurts, A. Noomen, A. Jellema, & M. A. J. S. Nan Boekel. 1999. 
"Dairy technology principles of milk properties and processes". 3nd ed. Marcel 
Dekker Inc. New York. p. 325- 608. 
Wang J. &L ying . 
2012. "Comparative Study of Chemical Composition and Texture 
Profile Analysis between Camembert Cheese and Chinese Sufu" Biotechnology 
Frontier Vol. 1, p. 1-8. 
Weber, F. 1987. Curd drainage Cheesemaking: Science and Technology. In Eck, A. p. 
22-36. 
Wendin, K., M. Langton, L. Caous & G. Hall. 2000. "Dynamic analysis of sensory 
and microstructural properties of cream cheese. " Food Chemistry. Vol. 71. p. 363-378. 
Weisburg, W., S. Barns, D. Pelletier, & D. Lane. 1991. "16S ribosomal DNA 
amplification for phylogenetic study". Journal of Bacteriology. Vol. 173 p. 697. 
Widyastuti, Y., Rohmatussolihat, & F. Andi.. 2014. "The Role of Lactic Acid Bacteria 
in Milk Fermentation", Food and Nutrition Sciences, Vol. 5. p. 435-442. 
Wouters, J. T. M., E. H. E. Ayad, J. Hugenholtz & G. I. Smit. 2002. "Microbes from 
Raw Milk for Fermented Dairy Products. " International Dairy Journal. Vol. 12. 
p. 91-109. 
131 
Wu, F. C., C. W. Chang & I. L. Shih. 2013. "Optimization of the production and 
characterization of milk clotting enzymes by Bacillus subtilis natto". SpringerPlus. 
Vol. 2. p. 33. 
Xing, G., D. Pan, M. Tong & X. Zeng. 2012. "Purification and characterization of 
cell-envelopeproteinase from Lactobacillus casei DI-1". African Journal of 
Biotechnology. Vol. 11. p. 15060-15067. 
Yegin, S., M. Fernandez-Lahore, U. Guvenc & Y. Goksungur. 2010. "Production of 
extracellular aspartic protease in submerged fermentation with Mucor mucedo DSM 
809". African Journal of Biotechnology. Vol. 38. p. 6380-6386. 
Yeung, P. S. Sanders M. M. E., Kitts C. L., Cano R. & Tong P. S. 2002. "Species- 
Specific Identification of Commercial Probiotic Strains. " Journal of Dairy Science. 
Vol. 85. p. 1039-1051. 
Yu, P. J. & C. C. Chou. 2005. "Factors affecting the growth and production of milk- 
clotting by Amylomyces rouxii in rice liquid medium". Food Technology and 
Biotechnology, Vol. 43. p. 283-288. 
Yuri, J. J., D. M. Barbano, & P. S. Kindstedt 1995. "Mozzarella cheese: impact of 
whey pH at draining on chemical composition, proteolysis and fimctional properties". 
Journal of Dairy Science. Vol. 78. p. 1-7. 
Zaurari, A., J. P. Accolas & M. J. Desmazeaud. 1992. "Metabolism and biochemical 
characteristics of yogurt bacteria: a review". Lait Vol. 72 p. 1-34 
Zhang, Z. G., C. Z. Wang, Z. Y. Yao, J. F. Zhao, F. X. Lu, G. M. Yu, W. J. Lan & Z. X. 
Lu. 2011. "Isolation and identification of a fungal strain QY229 producing milk- 
clotting enzyme". European Food Research and Technology, Vol. 232. p. 861-866. 
Zoetendal, E. G., A. D. L. Akkermans & W. M. De. Vos. 1998. "Temperature Gradient 
Gel Electrophoresis Analysis of 16S rRNA for Human Fecal Samples Reveals Stable 
and Host-Specific Communities of Active Bacteria". Applied and Environmental 
Microbiology. Vol. 64. p. 3854-3859. 
132 
APPENDIX 1 
Figure: Milk Clotting Activity of LAB Extracellular Proteinase 
Figure: Skim Milk coagulate by LAB Enzyme 
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APPENDIX 4 
Figure: Goat Milk Dadih 7d of Storage at 4°C 
Figure: Goat Milk Dadih 1d of Storage at 4 °C 
138 
APPENDIX 5 
Figure: Texture Profile plot of Skim Milk Dadih 
Figure Texture Profile plot of Goat Milk Dadih 
«ce Iti1 None 
........................  ............ o xs a. o na aao Time )sec) 
139 
APPENDIX 6 
Figure: A standard Curve of Bovine Serum Albumin (BSA) 
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140 
LIST OF PUBLICATION 
Imdakim, M. M., Zaiton H., Aween M. M., Elshaafi, I. M. and Belal J. M 2015. Milk 
clotting & Proteolytic Activity of Enzyme Preparation from Pediococcus acidilactici SH 
for Dairy Products African Journal of Biotechnology, Vol. 14 (2): P. 133-142. 
Mohamed, M. A. & Zaiton H. 2014. Characterization and Partial Purification of Milk 
Clotting Enzyme Produced by Pediococcus acidilactici SH and Lactobcilhus paracasei 
CFI 32nd Symposium of the Malaysian Society for Microbiology 6 to 8 December 2014 
Terengganu, Malaysia. 
Imdakim, M. M., Zaiton H., Elshaafi I. M. Lactic acid bacteria as source of milk clotting 
enzyme for soft cheese making. Student's Innovation Competiotion (SIC2012). Universiti 
Sains Islam Malaysia. 
Imdakim, M. M., Zaiton H., Microbial Rennet from Lactic acid bacteria. ITEX, 2012. 
Universiti Sains Islam Malaysia. 
Imdakim, M. M., Zaiton H., Abuaker M. A. S. 2012 Milk Clotting and Proteolytic Activity 
of Enzyme from Pediococcus acidilactici for soft cheese making. Ekspo Inovasi Islam 
20- 21 October Universiti Sains Islam Malaysia. 
Mohamed Muftah Ahmad and Zaiton Hassan. 2015. Detection of milk clotting enzyme 
produced by Pediococcus acidilactici and Lactobacillus paracasei. Proceeding of USIM 
Colloqiuni. Sepang. Malaysia. November 2015.