Pouya HassandarvishVunjia TiongNurul Azmawati MohamedHarsha ArumugamAbhishek AnanthanarayananMurtaza QasuriYacine HadjiatSazaly Abubakar2024-05-272024-05-272020-122021-2-9Hassandarvish, P., Tiong, V., Mohamed, N. et al. In vitro virucidal activity of povidone iodine gargle and mouthwash against SARS-CoV-2: implications for dental practice. Br Dent J (2020). https://doi.org/10.1038/s41415-020-2402-00007-0610900-4010.1038/s41415-020-2402-0https://oarep.usim.edu.my/handle/123456789/4103BRITISH DENTAL JOURNAL | ONLINE PUBLICATION | DECEMBER 10 2020INTRODUCTION Virus particles in respiratory droplets and aerosols generated during medical/dental procedures are a potential source of SARS-CoV-2 cross infection. In the dental setting, oral decontamination could be an important adjunct to personal protective equipment and is recommended by a number of national COVID-19 guidance documents for dental settings. AIM To assess the in vitro virucidal activity of an oral povidone iodine (PVP-I) product against SARS-CoV-2. MATERIAL AND METHODS BETADINE gargle and mouthwash (1% PVP-I) was tested against SARS-CoV-2 virus under both clean and dirty conditions using a suspension assay based on EN14476 methodology. Virucidal activity of the product, undiluted and at 1:2 dilution, was tested at contact times of 15, 30 and 60 seconds. Viral titres were calculated using the Spearman-Kärber method and reported as median tissue culture infectious dose (TCID50/ml). RESULTS The undiluted product achieved >5 log10 reduction in viral titres compared to the control at 15, 30 and 60 seconds under both clean and dirty conditions. At a twofold dilution (0.5% PVP-I), the test product demonstrated >4 log10 kill at 15 seconds and >5 log10 kill at 30 and 60 seconds in both clean and dirty conditions. CONCLUSION PVP-I gargle and mouthwash product, undiluted and at 1:2 dilution, demonstrated potent and rapid virucidal activity (≥4 log10 reduction of viral titre) in 15 seconds against SARS-CoV-2 in vitro. The PVP-I gargle and mouthwash product is widely available and could be readily integrated into infection control measures during dental treatment including pre-procedural oral decontamination.enArticle14-10