Mohd Rizal AbasAbdul Jalil Abdul KaderMohd Sahaid KhalilAidil Abdul HamidMohd Hafez Mohd Isa2024-05-282024-05-2820132010-4618313-610.7763/IPCBEE. 2013. V55. 17https://oarep.usim.edu.my/handle/123456789/46312013 International Conference on Food and Agricultural Sciences IPCBEE vol.55(2013)Surfactin, a lipopeptidic biosurfactant from Bacillus subtilis can only be produced under appropriate fermentation conditions and one of the factor being considered is their nutrient source. Conventionally, production of surfactin had been practised by utilizing commercial laboratory media in either both small and large scale fermentation. Alternative media options are being sought from agro-based wastes in order to minimize the production cost due to its relatively abundant and inexpensive raw materials. Palm oil mill effluent (POME), an agricultural waste from palm oil industry has been reviewed as a promising candidate that could potentially to be exploited. This study investigated on the feasibility of POME as fermentation media in surfactin production by using prominent surfactin producer of B. subtilis American Type Culture Collection(ATCC) 21332. Nutrient analysis showed POME consisted of significant amount of fermentable sugars, nitrogenous compounds, and essential elements that could support the bacterial growth and surfactin production. Fermentation study evaluated that POME media at various concentrations (10, 30, and 50%) were capable to produce surfactin with different yields. The highest surfactin amount was achieved by using 50 % (v/v) of POME compared to other concentrations studied. Keywords: Surfactin, lipopeptides, Bacillus subtilis ATCC 21332, palm oil mill effluent (POME), fermentationenSurfactin,lipopeptides,Bacillus subtilis ATCC 21332,palm oil mill effluent (POME),fermentationArticle87935517