Rahayu AhmadSiti Mariatul Usyida AliasAidil Abdul HamidWan Mohtar Wan YusoffEndom IsmailFauzi Daud2024-05-272024-05-2720152321-6883https://saspublisher.com/sajb-34/https://oarep.usim.edu.my/handle/123456789/3243Volume:3 Issue:4Production of extracellular and intracellular polysaccharides (EPS, IPS) from Lentinus squarrosulus mycelium was carried out through submerged fermentation. The one factor-at-a-time method was adopted to investigate the effect of medium components (carbon, nitrogen), and environmental factor (initial pH) on dry weight of mycelium and EPS concentration. Sucrose and yeast extract gave the highest EPS concentration with 3.56 mg/mL and 1.23 mg/mL respectively when carbon and nitrogen sources were employed. Combination of ammonium chloride and yeast extract (30:70) ratio increased the mycelium dry weight up to 10.8 g/L and EPS concentration of 4.39 mg/mL. An initial pH of 7.5 showed maximum EPS concentration of 3.82 mg/mL with mycelium dry weight of 7 g/L. Crude extracts from hot water and cold water extraction of Lentinus squarrosulus mycelium (IPS HWE, IPS CWE) and water extract from supernatant (EPS WE) was proved to have antiproliferative activity against A549 lung carcinoma cell lines (ATCC) as compared to ethanol extract.en-USpolysaccharides, Lentinus squarrosulus, mycelium, antiproliferative activityArticle37738534