T. A. M. OthmanRohazila Mohd HanafiahNoor Akmar NamShahida Mohd-SaidSiti Noor Adnalizawati Adnan2024-05-282024-05-28201920/12/20191309-100X1209-16chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/viewer.html?pdfurl=http%3A%2F%2Fwww.jidmr.com%2Fjournal%2Fwp-content%2Fuploads%2F2019%2F10%2F2-D18_693_Siti_Noor_Adnalizawati_Adnan.pdf&clen=489379&chunk=truehttps://oarep.usim.edu.my/handle/123456789/4221(J Int Dent Med Res 2019; 12(3): 848-853)The potential antimicrobial properties of tannins from Phyllanthus columnaris stem bark were evaluated against three Gram-positive cariogenic bacteria (Streptococcus salivarius ATCC 13419, Streptococcus oralis ATCC 6249 and Streptococcus mutans ATCC 25175), two obligate anaerobic Gram-negative periodontopathic bacteria (Porphyromonas gingivalis ATCC 33277, and Fusobacterium spp ATCC 25586), and five Candida spp (C. albicans ATCC 14053, C. parapsilosis ATCC 22019, C. tropicalis ATCC 750, C. krusei ATCC 6258 and C. glabrata ATCC 2001). Antimicrobial activities of tannins were determined using the disc diffusion test as well as minimum inhibitory concentration and minimum bactericidal/fungicidal concentration tests. The chemical composition of tannins was analysed by direct infuse mass spectrometry. Tannins inhibited the growth of all tested pathogens at MIC value ranging from 0.16 to 1.25 mg/mL. All tested bacteria showed similar higher level of susceptibility against tannins as the lowest MIC value was detected (0.16 mg/mL) except for S. oralis (0.63 mg/mL). However, both Gram negative anaerobes recorded the lowest MBC value at 0.32 mg/mL as compared to the Gram positive cariogenic bacteria (2.5->5.0 mg/mL). DIMS analysis of precursor ions detected four compounds present in tannins namely punicafolin, (S)-Nerolidol 3-O-[a-L-Rhamnopyranosyl-(1->4)-a-L-rhamnopyranosyl-(1->2)-[4-(4-hydroxy-3 methoxycinnamoyl)-(E)-a-L-rhamnopyranosyl-(1->6)]-b-D-glucopyranoside], ent-Epicatechin-(4alpha->8)-ent-epicatechin-(4alpha->8)-ent-epicatechin 3',3''-digallate and pavetannin C1 respectively.enOral pathogens,Tannins,Antimicrobial, .DIMSArticle848853123