Sabullah, MKMKSabullahRahman, MFMFRahmanAhmad, SASAAhmadSulaiman, MRMRSulaimanShukor, MSMSShukorShamaan, NANAShamaanShukor, MYMYShukor2024-05-292024-05-2920160126-053710.17503/agrivita.v38i1.654WOS:000376560000001https://oarep.usim.edu.my/handle/123456789/12053Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molybdenum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing bacterial resting cells in a microplate format. The bacterium reduces molybdate to Mo-blue optimally between pH 6.3 and 6.8 and at 34 degrees C. Glucose was the best electron donor for supporting molybdate reduction followed by lactose, maltose, melibiose, raffinose, d-mannitol, d-xylose, I-rhamnose, I-arabinose, dulcitol, myo-inositol and glycerol in descending order. Other requirements include a phosphate concentration at 5.0 mM and a molybdate concentration between 20 and 30 mM. The molybdenum blue exhibited an absorption spectrum resembling a reduced phosphomolybdate. Molybdenum reduction was inhibited by mercury, silver, cadmium and copper at 2 ppm by 45.5, 26.0, 18.5 and 16.3%, respectively. Biochemical analysis identified the bacterium as Klebsiella oxytoca strain Saw-5. To conclude, the capacity of this bacterium to reduce molybdenum into a less toxic form and to grow on glyphosate is novel and makes the bacterium an important instrument for bioremediation of these pollutants.en-USbioreductionglyphosateKlebsiella oxytocamolybdenum bluemolybdenum-reducing bacteriumArticle113381