Fatin Hamimi MustafaIrneza IsmaiAhmad Aiman Zuhaily Ahmad MunawarBasmah Abdul BasirRafidah Hanim ShuebAhmad Adebayo IrekeolaWan Zakiah Wan IsmailJuliza JamaludinSharma Rao BalakrishnanMus’ab SahrimNik Yusnoraini Yusof2024-06-162024-06-1620232023-12-18Fatin Hamimi Mustafa,Irneza Ismail,Ahmad Aiman Zuhaily Ahmad Munawar,Basmah Abdul Basir,Rafidah Hanim Shueb,Ahmad Adebayo Irekeola,Wan Zakiah Wan Ismail,Juliza Jamaludin,Sharma Rao Balakrishnan,Mus’ab Sahrim,Nik Yusnoraini Yusof (2023). A review on current diagnostic tools and potential optical absorption spectroscopy for HFMD detection. Analytical Biochemistry, 683, 115368. https://doi.org/10.1016/j.ab.2023.1153680003-26972073-1510.1016/j.ab.2023.115368.https://oarep.usim.edu.my/handle/123456789/19175Analytical Biochemistry Volume 683 Page (1-10)Hand, Foot, and Mouth Disease (HFMD) is an outbreak infectious disease that can easily spread among children under the age of five. The most common causative agents of HFMD are enterovirus 71 (EV71) and coxsackievirus A16 (CVA16), but infection caused by EV71 is more associated with fatalities due to severe neurological disorders. The present diagnosis methods rely on physical examinations by the doctors and further confirmation by laboratories detection methods such as viral culture and polymerase chain reaction. Clinical signs of HFMD infection and other childhood diseases such as chicken pox, and allergies are similar, yet the genetics and pathogenicity of the viruses are substantially different. Thus, there is an urgent need for an early screening of HFMD using an inexpensive and user-friendly device that can directly detect the causative agents of the disease. This paper reviews current HFMD diagnostic methods based on various target types, such as nucleic acid, protein, and whole virus. This was followed by a thorough discussion on the emerging sensing technologies for HFMD detection, including surface plasmon resonance, electrochemical sensor, and surface enhanced Raman spectroscopy. Lastly, optical absorption spectroscopic method was critically discussed and proposed as a promising technology for HFMD screening and detection.en-USHandfootand mouth disease (HFMD) Outbreak Enterovirus 71 (EV71) Coxsackievirus A16 (CVA16) Diagnotic tools Biosensors Optical absorption spectroscopyJurnal110683115368