Masniza, M. L.M. L.MasnizaZetty Nadia Mohd ZainNur Syahrina RahimHayati, A. R.A. R.HayatiWirda, A. A. AsralA. A. AsralWirdaFauzi, M. Fadlul AzimM. Fadlul AzimFauziFariha, M. M. NurM. M. NurFariha2024-05-292024-05-292020Masniza, M.L. & Nadia, M.Z. & Rahim, Nur & Hayati, A.R. & Asnawi, Asral & Mansur, Fadlul & Fariha, Nur. (2020). Date palm fruit (var. Ajwa) promotes proliferation of human bone marrow mesenchymal stem cells: potential natural booster for endogenous stem cells growth. Fruits. 75. 161-169. 10.17660/th2020/75.4.3.0248-12941625-967X online10.17660/th2020/75.4.3WOS:000579370300003https://www.pubhort.org/fruits/75/4/3/index.htmhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85090893339&doi=10.17660%2fth2020%2f75.4.3&partnerID=40&md5=eba141abe804e52b5864d629640a23b6https://oarep.usim.edu.my/handle/123456789/11973Fruits, The International Journal of Tropical and Subtropical Horticulture Fruits 75 (4) 161-169 ISSN 0248-1294 print and 1625-967X onlineObjective - Maintenance of bone marrow derived mesenchymal stem cells (BMMSC) numbers is important to preserve the balance required for normal tissue regeneration. 'Ajwa' date palm fruit, a popular Middle Eastern food, is known for its superior nutrition and medicinal properties. However, the direct effect of 'Ajwa' dates on stem cell biology remains unclean This study aims to investigate the potential effect of 'Ajwa' date palm fruit on BMMSC proliferation. Materials and methods - BMMSC were treated with different concentrations of aqueous extract of Ajwa' date palm fruit (mg mL(-1)). Proliferation, morphology, surface markers and gene expression of BMMSC were evaluated after the treatment. Results and discussion - 'Ajwa' date palm fruit significantly enhanced BMMSC proliferation and retained the sternness of BMMSC. Quantitative gene expression analysis demonstrated significant increase of proliferation related genes; beta-Catenin, C-Myc and HGF and lower expression of apoptotic genes; BAX and Caspase-3 and senescence-related gene, p21. Conclusion - These findings suggest the potential effect of 'Ajwa' date palm fruit as stem cell nutrition in the preservation of endogenous BMMSC for tissue regeneration.en-USPhoenix dactylifera L.gene expressionnutritionstem cell growthArticle161169754