Othman S.S.Basri M.Hussein M.Z.Abdul Rahman M.B.Rahman R.N.Z.Abd.Salleh A.B.Jasmani H.2024-05-292024-05-292008308814610.1016/j.foodchem.2007.04.0262-s2.0-34548292441https://www.scopus.com/inward/record.uri?eid=2-s2.0-34548292441&doi=10.1016%2fj.foodchem.2007.04.026&partnerID=40&md5=84cd147ff0dde7c53d9c4d4562460fbfhttps://oarep.usim.edu.my/handle/123456789/9863Optically active (-)-menthyl butyrate was synthesized by enantioselective esterification of racemic (±)-menthol and butyric anhydride using lipase from Candida rugosa immobilized onto epoxy-activated supports of Eupergit C and Eupergit C 250 L through physical adsorption method. The effects of various temperature, storage condition, stability in organic solvent and lipase recyclability were investigated for their influence on the enzymatic enantioselective formation of (-)-menthyl butyrate. The immobilized lipases retained high catalytic activity of up to 31% yield and 100% enantiomeric excess of the desired product, and showed better stability compared to the native lipase. They also exhibited about 50% retained activity even after incubation at higher temperatures, storage at room temperature and after long incubation in hexane. Immobilized lipases also showed considerably efficient reusability.en-USEnantioselectivityEpoxideImmobilizationLipaseMenthyl butyrateArticle4374431062FOCHD