Ong E.B.B.Anthony A.A.Ismail A.Ismail A.Lim T.S.2024-05-282024-05-282013732889310.1016/j.diagmicrobio.2013.05.0102-s2.0-84881544694https://www.scopus.com/inward/record.uri?eid=2-s2.0-84881544694&doi=10.1016%2fj.diagmicrobio.2013.05.010&partnerID=40&md5=6c9793de23d716da35ad8743cd9e0f66https://oarep.usim.edu.my/handle/123456789/892123790417The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using sera from typhoid patients showed the presence of HlyE-specific antibodies in circulation. � 2013 Elsevier Inc.en-USAntigenHlyEImmunoblot, ELISASalmonellaTyphiArticle8789771DMIDD