Nordin N.Majid N.A.Othman R.Omer F.A.A.Nasharuddin M.N.A.Hashim N.M.2024-05-282024-05-2820181360818510.1007/s10495-018-1447-x2-s2.0-85041904621https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041904621&doi=10.1007%2fs10495-018-1447-x&partnerID=40&md5=f282e66f7aa0f410eb747d6b88adfee1https://oarep.usim.edu.my/handle/123456789/929729430581Plagioneurin B belongs to acetogenin group has well-established class of compounds. Acetogenin group has attracted worldwide attention in the past few years due their biological abilities as inhibitors for several types of tumour cells. Plagioneurin B was isolated via conventional chromatography and tested for thorough mechanistic apoptosis activity on human ovarian cancer cells (CAOV-3). Its structure was also docked at several possible targets using Autodock tools software. Our findings showed that plagioneurin B successfully inhibits the growth of CAOV-3 cells at IC50 of 0.62��M. The existence of apoptotic bodies, cell membrane blebbing and chromatin condensation indicated the hallmark of apoptosis. Increase of Annexin V-FITC bound to phosphatidylserine confirmed the apoptosis induction in the cells. The apoptosis event was triggered through the extrinsic and intrinsic pathways via activation of caspases 8 and 9, respectively. Stimulation of caspase 3 and the presence of DNA ladder suggested downstream apoptotic signalling were initiated. Further confirmation of apoptosis was conducted at the molecular levels where up-regulation in Bax, as well as down-regulation of Bcl-2, Hsp-70 and survivin were observed. Plagioneurin B was also seen to arrest CAOV-3 cells cycle at the G2/M phase. Docking simulation of plagioneurin B with CD95 demonstrated that the high binding affinity and hydrogen bonds formation may explain the capability of plagioneurin B to trigger apoptosis. This study is therefore importance in finding the effective compound that may offer an alternative drug for ovarian cancer treatment. � 2018, Springer Science+Business Media, LLC, part of Springer Nature.en-USAcetogeninApoptosisCAOV-3 cellsOvarian cancerPathwaysPlagioneurin BArticle152169232APOPF