Noraziah NordinSyam MohanNajihah Mohd. Hashim2024-05-282024-05-282014Noraziah Nordin, and Syam Mohan, and Najihah Mohd. Hashim, and Asdren Zajm, and Noor Shafifiyaz Mohd. Yazid, and Mashitoh Abdul Rahman, and Fatima Abdelmutaal Ahmed Omer, and Hanita Omar, and Fatimah A'fifah Alias, and Hapipah Mohd. Ali, (2014) Antioxidant, anticancer and antimicrobial activities of methanolic extracts from enicosanthellum pulchrum (King) heusden. Sains Malaysiana, 43 (10). pp. 1515-1521. ISSN 0126-60390-02169-10http://www.ukm.my/jsm/english_journals/vol43num10_2014/vol43num10_2014p1515-1521.htmlhttps://oarep.usim.edu.my/handle/123456789/5730Biological activities of crude methanolic extracts from leaves, barks, twigs and roots ofEnicosanthellum pulchrum were investigated in four bioassays. The antioxidant, 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging assay showed that bark and twig extracts showed high inhibitory activity with 60 and 56% inhibition at 1 mglmL and IC50values of 0.43 t 0.04 and 0.64 t 0.05 mglmL, respectively. The bark and root extracts showed greater reducing power (FRAP) than several standard drugs used in the bioassay. Methanolic extracts of leaves, twigs and roots displayed strong cytotoxicity to breast cancer cell line (MCF-7), myelomonocytic leukaemia cell line (WEHI-3) and ovarian cancer cell line (CAOV-3); the IC50 of the leaf extract were 7.8 t 0.85 pg/mL (MCF-7) and 9.0 t 0.13 pgImL (WEHI-3), while those for the twig and root extracts were 13.9 t 0.35 and 7.3 t 0.98 pg/mL (CAOV-3), respectively. In the antimicrobial assays, the extracts were tested against ten bacterial strains and two fimgal strains. Bark and twig extracts displayed high inhibitory activity to Bacillus subtilis with 13.3 t 0.57 and 12.0 t 0.01 mm inhibition, respectively. In addition, the twig extract displayed better minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) compared with the bark extract (MIC 0.5 and 1.0 mg/mL, MBC 1.0 and 2.0 mg/mL, respectively). For antifitngal activity, all extracts showed inhibition on Candida albicans but not on Aspergillus niger. The obtained results suggested that this plant may possibly contain bioactive compounds in the active extracts.enAnticancer, antimicrobial, antioxidant, Enicosanthellum pulchrum, methanolic extractsArticle1515–152104310