Syarifah Amni Safiya Syed Nahar AfendiNur Auni Raudhah Binti Mohamad FarizLiyana Amalina AdnanNurdalila A’wani Abdul Aziz2025-11-172025-11-172025Syarifah Amni Safiya Syed Nahar Afendi, Nur Auni Raudhah Binti Mohamad Fariz, Liyana Amalina Adnan, Rahayu Ahmad, A’wani Aziz Nurdalila. (2025). Sustainability Bioethanol Production From Agricultural Waste With Valorisation Of Fermentation Residues In Microbial Fuel Cells. Insan Junior Researcher International Conference & Innovation (IJURECON) 2025, 163–169. https://raudahusim.sharepoint.com/:b:/s/USIM-eDocs/EfVkn9zP4m9HniZRvI2XhQMBlU12RcfdxRZHlzdrKyiznQ?e=syZY7bhttps://raudahusim.sharepoint.com/:b:/s/USIM-eDocs/EfVkn9zP4m9HniZRvI2XhQMBlU12RcfdxRZHlzdrKyiznQ?e=syZY7bhttps://oarep.usim.edu.my/handle/123456789/27941Insan Junior Researcher International Conference & Innovation (iJURECON) 2025 : “STREAM for a Better Future/ editor : Ahmad Fuad Mohamad Amin, Nurul Shazwani Binti Mohamed, Rossidi Bin Usop, Abdel Rahman Ibrahim Suleiman Islieh Organised by Kolej PERMATA Insan 10-11 October 2025Bioethanol is a renewable fuel produced from the fermentation of sugars and starches in plant-based biomass. However, large-scale production generates considerable wastes, which, if untreated, may cause environmental concerns. These by-products can instead serve as substrates for microbes in microbial fuel cells (MFCs), bioelectrochemical systems that generate electricity through microbial metabolism. This study aims to reduce environmental pollution by developing a hybrid system that integrates two processes: (i) bioethanol production from agricultural residues and (ii) MFC operation utilizing the resulting wastes. Rice straw was selected as feedstock. Pleurotus ostreatus (oyster mushroom) degraded the lignocellulosic structure to release fermentable sugars, which were fermented by Saccharomyces cerevisiae (yeast) into bioethanol and CO₂. The ethanol was distilled, while byproducts (thin stillage, yeast autolysate) were supplied to the MFC. In the anode chamber (anaerobic), Shewanella oneidensis. consumed the wastes and generated electrons which facilitated electrical generation, while protons diffused through a proton exchange membrane. In the cathode chamber (aerobic), Bacillus subtilis facilitated the reduction reaction. Electrical output was measured using a multimeter across the anode and cathode, followed by load application with known resistors. The hybrid system produced bioethanol with a yield of ~0.22 g g⁻¹ dry rice straw and generated an open circuit voltage of ~0.60 V and current output of 0.6 mA (power density ~36 mW m⁻²). These results demonstrate the feasibility of coupling bioethanol production with MFCs, providing dual benefits: renewable fuel generation and sustainable electricity recovery. This approach reduces agricultural waste, promotes waste-toenergy conversion, and contributes to lowering environmental pollution.en-USBioethanolMicrobial Fuel Cell (MFC)Rice strawShewanellaBacillus subtilistext::conference output::conference proceedings::conference paper163169