Noor H.M.Muhamad H.S.Ismail I.N.A.Radzi S.M.Rehan M.M.Kader A.J.A.Mohamad R.2024-05-292024-05-292014H. Mohd Noor, H. Muhamad, I. Ismail, A. Shukri, S. Mat Radzi, M. Rehan, A. Kader, "Antimicrobial Protein Produced by Lactobacillus plantarum ATCC8014 in the Presence of Cymbopogon Sp. Essential Oils", Applied Mechanics and Materials, Vol. 754-755, p. 1045, 2015 DOI: https://doi.org/10.4028/www.scientific.net/AMM.754-755.1045978304000000010139826https://doi.org/10.4028/www.scientific.net/KEM.594-595.3702-s2.0-84891864682WOS:000336337200067https://www.scopus.com/inward/record.uri?eid=2-s2.0-84891864682&doi=10.4028%2fwww.scientific.net%2fKEM.594-595.370&partnerID=40&md5=d79306fec8b952624d28f0773fcb53e4https://www.scientific.net/KEM.594-595.370https://oarep.usim.edu.my/handle/123456789/9914Proteins levels produced by bacteria may be increased in stressful surroundings, such as in the presence of antibiotics. It appears that many antimicrobial agents or antibiotics, when used at low concentrations, have in common the ability to activate or repress gene transcription, which is distinct from their inhibitory effect. There have been comparatively few studies on the potential of antibiotics or natural compounds in nature as a specific chemical signal that can trigger a variety of biological functions. Therefore, this study was focusing on the effect of essential oil from Cymbopogon flexuosus in regulating proteins production by Bacillus subtilis ATCC21332. The Minimum Inhibition Concentration (MIC) of the C. flexuosus essential oil on B. subtilis was determined by using microdilution assay, resulting 1.76mg/ml. The bacteria cells were further exposed to the C. flexuosus essential oil at concentration of 0.01 MIC for 72 h. The proteins were then isolated and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Proteins profile showed that a band with approximate size of 30 kDa was appeared for the treated bacteria with C. flexuosus essential oil. Thus, B. subtilis ATCC21332 in stressful condition with the presence of C. flexuosus essential oils at low concentration could induce the protein production. The isolated protein also showed antimicrobial activity against selected Gram-positive and Gram-negative bacteria. Copyright � 2014 Trans Tech Publications Ltd, Switzerland.enBacillus subtilis ATCC21332Cymbopogon flexuosus essential oilProteinAnti-microbial activityBacillus SubtilisBiological functionsCymbopogonGene transcriptionsGram-negative bacteriaMinimum inhibition concentrationsSodium dodecyl sulfate-polyacrylamide gel electrophoresisAntibioticsBacteriaElectrophoresisProteinsSodium dodecyl sulfateTranscriptionEssential oilsArticle370377594-595KEMAE