Muhammad Fadzlisyam RedzuanIrfan Danial IsmadiMuhammad Fakhrullah Mohamad AzmiIzzah Afifah IbrahimMohd Ifwat Mohd GhazaliNurul Azmawati Mohamed @ Abd AzizNizam TamchekShahino Mah Abdullah2025-08-052025-08-052025Muhammad Fadzlisyam Redzuan, Irfan Danial Ismadi, Muhammad Fakhrullah Mohamad Azmi, Izzah Afifah Ibrahim, Mohd Ifwat Mohd Ghazali, Nurul Azmawati Mohamed, Nizam Tamchek, & Shahino Mah Abdullah. (2025). Fabrication of 3D-Printed Flow Cell Biosensor for Pathogenic Escherichia Coli Bacteria Detection. International Conference and Innovation on Education, Science & Technology (ICIEST 2025), 68–75.978-629-95846-0-5https://flip.usim.edu.my/permatainsan/iciest2025/Proceeding-ICIEST-2025.pdfhttps://oarep.usim.edu.my/handle/123456789/27356International Conference And Innovation On Education, Science & Technology (ICIEST 2025) “Fostering Innovation And Creativity Through Education, Science & Technology” 21st June 2025 Editor : Hatika Kaco, Diani Mardiana Mat Zin, Fadzidah Mohd Idris, Siti Munirah Mohd Nurhidaya Mohamad Jan, Nor Atirah Izzah Zulkefli, Nadiah Suboh Organised by : Education & Advanced Sustainability (EdAS) unit, Kolej PERMATA Insan, Universiti Sains Islam MalaysiaThis research seeks to develop an innovative 3D-printed biosensor for the rapid and accurate detection of Escherichia coli (E. coli) bacteria in water samples. The proposed biosensor will incorporate a flow cell design, which allows continuous monitoring and efficient detection of E. coli in real-time. The sensor will be fabricated using conductive polymer-based materials integrated with specific biological recognition elements to ensure high sensitivity and specificity. The 3D printing technology will be utilized to create a precise and reproducible flow cell structure, optimizing the sensor's functionality and scalability. The research will proceed through several key phases: designing and simulating the flow cell structure, selecting and functionalizing the sensing materials, fabricating the biosensor using 3D printing techniques, and conducting extensive testing with water samples containing various concentrations of E. coli. This research will establish a foundation for future advancements in portable and effective biosensing devices for early detection of bacterial contamination.en-USEscherichia ColiBiosensorFlow Cell3D printingtext::conference output::conference proceedings::conference paper6875