Huda-Faujan N.Rahim Z.A.Rehan M.M.Ahmad F.B.H.2024-05-292024-05-292015139425062-s2.0-84932121940https://www.scopus.com/inward/record.uri?eid=2-s2.0-84932121940&partnerID=40&md5=831d4ad5dcc297aa82505455f2e97356https://oarep.usim.edu.my/handle/123456789/9918Malaysian Journal of Analytical Sciences, Vol 19No 3(2015): 611 -624Vegetables have been believed to exhibit antioxidant activities due to its phenolic content. Thus, this study was carried out to determine the total phenolic content of water and ethanolic extracts of Malaysian traditional vegetables and assess their antioxidant activities. Eight samples of Malaysian traditional vegetables were dried and extracted its phenolic compounds using water and ethanolic solvent. Total phenolic content of the extracts were compared and evaluated using Folin-Ciocalteu and PrussianBlue reagent. The antioxidant activity were assessed using ferric thiocyanate assay and DPPH free radical scavenging assays. Results found that total phenolic content of water extracts ranged from 7.08 to 14.76 mg GAE (Folin-Ciocalteu assay) and 3.50 to7.82 mg GAE (Prussian Blue assay). However, the content of phenolic of ethanolic extracts ranged from 5.21 to 15.86 mg GAE (Folin-Ciocalteu assay), and 1.84 to 11.54 mg GAE (Prussian Blue assay). The highest antioxidant activity was observed in water extracts of Etlingera elatior(75.6%) and ethanolic extracts of Sauropus androgynus(78.1%). Results also found that the best half maximal inhibitory concentration or IC50were demonstrated by water and ethanolic extracts of Sauropus androgynus which demonstrated 0.077 mg/mL and 0.078 mg/mL, respectively. Hence, this study obtained that most of the Malaysian traditional vegetables have a potential source of natural antioxidant. Keywords:Folin-Ciocalteu method, Prussian Blue assay, antioxidant activities, Malaysian vegetablesen-USAntioxidant activitiesFolin-ciocalteu methodMalaysian vegetablesPrussian blue assayArticle611624193