Mashri Ahmad Yahia,Mashri Ahmad YahiaMusa Ahmad,Musa AhmadChe Wan Zanariah Che Wan Ngah,Che Wan Zanariah Che Wan NgahLee Yook Heng2024-05-282024-05-282015--Ahmad Yahia, Mashri, Musa Ahmad, Che Wan Zanariah Che Wan Ngah, and Lee Yook Heng. “Determination of Hydroxymethylfurfural (HMF) in Honey Based on p-Toluidine as a Reagent Using Spectrophotometric Method.” Advanced Materials Research 1134 (December 2015): 28–33. https://doi.org/10.4028/www.scientific.net/amr.1134.28.1662-8985640-14https://doi.org/10.4028/www.scientific.net/AMR.1134.28https://www.scientific.net/AMR.1134.28https://oarep.usim.edu.my/handle/123456789/4210Spectrophotometric determination of hydroxymethylfurfural (HMF) based on p-toluidine as a reagent has been studied. The absorption intensities were measured at a wavelength range of 250 to 500 nm to record the absorption spectra before and after reaction with the HMF. The solution response was obtained at pH 3. The concentration of HMF was 5 × 10-3 M, whereas for p-toluidine was 8 × 10-3 M .The relative standard deviation (R.S.D.) of reproducibility was 3.33% when concentration of p-toluidine 3 × 10-3 M was used .The photostability was found to be good with 1.9 %. M was used . The photostability was found to be good with 1.9 %.enDetermination of Hydroxymethylfurfural (HMF) in Honey Based on p-Toluidine as a Reagent Using Spectrophotometric MethodArticle28331134