Salina Mat RadziNurul Jannah Abd RahmanHanina Mohd NoorN. Ariffin2024-05-282024-05-282014S. Mat Radzi, N. J. Abd Rahman, H. Mohd Noor, & N. Ariffin. (2014). Enzymatic Synthesis of Olive-Based Ferulate Esters: Optimization by Response Surface Methodology. International Journal of Chemical and Molecular Engineering, 8.0(8). https://doi.org/10.5281/zenodo.2828939849-3010.5281/zenodo.1094130https://publications.waset.org/9998973/enzymatic-synthesis-of-olive-based-ferulate-esters-optimization-by-response-surface-methodologyhttps://oarep.usim.edu.my/handle/123456789/4769Volume: 8 No:8Ferulic acid has widespread industrial potential by virtue of its antioxidant properties. However, it is partially soluble in aqueous media, limiting their usefulness in oil-based processes in food, cosmetic, pharmaceutical, and material industry. Therefore, modification of ferulic acid should be made by producing of more lipophilic derivatives. In this study, a preliminary investigation of lipase-catalyzed trans-esterification reaction of ethyl ferulate and olive oil was investigated. The reaction was catalyzed by immobilized lipase from Candida antarctica (Novozym 435), to produce ferulate ester, a sunscreen agent. A statistical approach of Response surface methodology (RSM) was used to evaluate the interactive effects of reaction temperature (40-80°C), reaction time (4-12 hours), and amount of enzyme (0.1-0.5 g). The optimum conditions derived via RSM were reaction temperature 60°C, reaction time 2.34 hours, and amount of enzyme 0.3 g. The actual experimental yield was 59.6% ferulate ester under optimum condition, which compared well to the maximum predicted value of 58.0%enFerulic acid, Enzymatic Synthesis, Esters, RSM.Article77077388