Nurdalila A’wani Abdul AzizMuhammad Aqil YazmiLiyana Amalina AdnanAhmad Bazli Ahmad Hilmi2025-11-172025-11-172025A'wani Aziz Nurdalila, Muhammad Aqil Yazmi, Liyana Amalina Adnan, Ahmad Bazli Ahmad Hilmi. (2025). Unveiling The Dental Benefits Of Citrus Hystrix And Lagenaria Siceraria Compounds Through Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy. Insan Junior Researcher International Conference & Innovation (IJURECON) 2025, 98–103. https://raudahusim.sharepoint.com/:b:/s/USIM-eDocs/EfVkn9zP4m9HniZRvI2XhQMBlU12RcfdxRZHlzdrKyiznQ?e=syZY7bhttps://raudahusim.sharepoint.com/:b:/s/USIM-eDocs/EfVkn9zP4m9HniZRvI2XhQMBlU12RcfdxRZHlzdrKyiznQ?e=syZY7bhttps://oarep.usim.edu.my/handle/123456789/27929Insan Junior Researcher International Conference & Innovation (iJURECON) 2025 : “STREAM for a Better Future/ editor : Ahmad Fuad Mohamad Amin, Nurul Shazwani Binti Mohamed, Rossidi Bin Usop, Abdel Rahman Ibrahim Suleiman Islieh Organised by Kolej PERMATA Insan 10-11 October 2025In this era of globalisation, many individuals with sensitivities to industrial medications and synthetic chemicals are increasingly seeking organic and environmentally friendly alternatives. This growing trend reflects a shift towards sustainable and eco-conscious choices in personal and healthcare products. This study investigates Citrus hystrix and Lagenaria siceraria, two plants traditionally used for their anti-inflammatory and antimicrobial properties, as potential natural alternatives for oral healthcare. The plant materials were dried at 60°C for 24 hours and ground into fine powder. Extracts were prepared using ethyl acetate combined with ethanol and hexane. Analysis using Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) identified several key functional groups linked to therapeutic effects. In Citrus hystrix, significant peaks were observed at 1733 cm⁻¹ (C=O stretch, esters), 1380 cm⁻¹ (NO₂ stretch, nitro compounds) . In Lagenaria siceraria, prominent peaks appeared at 1718 cm⁻¹, 1380 cm⁻¹, and 3309 cm⁻¹, reflecting similar bioactive groups. These functional groups are commonly associated with antimicrobial and anti-inflammatory activities. Literature supports their role in inhibiting oral pathogens and reducing inflammation, making them suitable for natural oral care formulations. In conclusion, the combination of traditional medicinal knowledge and modern analytical methods provides scientific support for the use of Citrus hystrix and Lagenaria siceraria as safe, plant-based alternatives to chemical oral treatments. This research emphasises the potential of natural bioresources in promoting sustainable, effective solutions for contemporary health challengesen-USCitrus hystrixLagenaria sicerariaATR-FTIRbioactive compoundsgum disease.text::conference output::conference proceedings::conference paper98103