Jalil Kader,Jalil KaderHanina Mohd Noor,Hanina Mohd NoorSalina Mat Radzi,Salina Mat RadziNur Aishah Abdul Wahab2024-05-272024-05-272013International Journal of Medicinal Plant Research ISSN: 2169-303X Vol. 2 (4), pp. 209-214, July, 2013.ISSN: 2169-303X-0849-17https://www.internationalscholarsjournals.org/journal/ijmpr/articles/antibacterial-activities-and-phytochemical-screening-of-the-acetone-extract-from-euphorbia-hirtahttps://oarep.usim.edu.my/handle/123456789/3964Euphorbia hirta is widely used as traditional medicine. The aim of the present study was to evaluate E. hirta parts and whole plants for the antibacterial activities plus phytochemical exist through acetone extracts. Antibacterial assay was carried out via agar well diffusion assay for screening purpose and finally through micro dilution method in order to determine minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) values against 10 selected bacteria. Gram positive bacteria were more susceptible compared to Gram negative bacteria via agar well diffusion assay. Whole plants E. hirta extracts very useful for inhibitory bacterium purpose. Whilst, all E. hirta extracts exhibited the bactericidal effect towards the ten bacteria tested. All bacteria tested susceptible to the roots extract based on the MBC/MIC value which is less than or equal to 4 (≤4). Phytochemical screening of E. hirta extracts revealed the presence of carbohydrates, lipids, protein, flavonoids, alkaloids, saponins, resins, steroids, acidic compounds, tannins, glycosides, phenols and terpenoids. Key words: Euphorbia hirta, antibacterial activities, agar well diffusion assay, micro dilution method, phytochemical screening.enEuphorbia hirta,antibacterial activities,agar well diffusion assay,micro dilution method,phytochemical screening.Article20921424