A'wani Aziz NurdalilaMuhammad ‘Ammar ‘Ayyasy Muhamad ZuhdiLiyana Amalina AdnanAhmad Bazli Ahmad Hilmi2025-10-212025-10-212024A'wani Aziz Nurdalila, Muhammad ‘Ammar ‘Ayyasy Muhamad Zuhdi, Liyana Amalina Adnan & Ahmad Bazli Ahmad Hilmi. (2024). Hexane-Acetone Extraction Techniques for Gas Chromatography-Mass Spectrometry. Insan Junior Researchers International Conference 2024 (IJURECON 2024) , 58–61. https://raudahusim.sharepoint.com/:b:/s/USIM-eDocs/EXrJQXKFR1JAurDWj_VmLiYBRXCK10uGRWJC_WRAu3bsBw?e=07OjRG3083-8509https://raudahusim.sharepoint.com/:b:/s/USIM-eDocs/EXrJQXKFR1JAurDWj_VmLiYBRXCK10uGRWJC_WRAu3bsBw?e=07OjRGhttps://oarep.usim.edu.my/handle/123456789/278465th Insan Junior Researchers International Conference 2024 (iJURECON 2024) : "STREAM for a better future”/ editors : Ahmad Fuad Mohamad Amin, Ahmad Bazli Ahmad Hilmi, Nurhidaya Mohamad Jan, Norazlina Subani 25-26 October 2024 Organized by : Kolej Permata InsanAt present, a lot of dental care products are mixtures of drugs and foreign substances. Some people are allergic or sensitive to industrial medications and synthetic chemicals. Many of those affected by this seek more organic and eco-friendly substitutes that do not cause allergic reactions, preferring natural alternatives. In this case, we want to make an alternative by comparing the compounds that are contained in industrial medications with eco-friendly substitutes by using Gas Chromatography-Mass Spectrometry (GC-MS). An acetone is used when preparing the extraction. We have found that Brucea javanica, Solanum lasiocarpum, and Alum Stone mixture contains compounds that can be used as an alternative. Brucea javanica is believed to offer anti-cancer, antimalarial, and anti-inflammatory benefits. Solanum lasiocarpum is widely used in traditional medicine as a diuretic and for treating various diseases such as fever, inflammation, and cough. Alum stone has antiseptic properties that help to fight against tooth decay. The dry samples were ground into a fine powder, accurately weighed, and placed in a conical flask containing a hexane mixed with acetone solvent system at a 4:1 ratio. The flask was then sealed with aluminum foil and allowed to sit overnight to ensure complete extraction. The mixture was subsequently filtered, separating the solid residue from the solvent. The extracted compounds were concentrated into an oil extract using a rotary evaporator set at 76°C and 264 atmosphere (ATM). A solvent to sample ratio was employed utilizing 10mLg-1 of sample. This extraction method will be applied in subsequent GCMS analysis.en-USBrucea JavanicaSolanum Lasiocarpumalum stoneGCMShexane-acetoneextraction techniquetext::conference output::conference proceedings::conference paper5861