Hanita OmarNajihah Mohd. HashimAsdren ZajmiNoraziah NordinSiddiq Ibrahim AbdelwahabAinnul Hamidah Syahadah AzizanA. Hamid A. HadiHapipah Mohd Ali2024-05-282024-05-2820131420-304910.3390/molecules18088994https://www.mdpi.com/1420-3049/18/8/8994https://oarep.usim.edu.my/handle/123456789/6547Volume 18 Issue 8The oxoaporphine alkaloid lysicamine (1), and three proaporphine alkaloids, litsericinone (2), 8,9,11,12-tetrahydromecambrine (3) and hexahydromecambrine A (4) were isolated from the leaves of Phoebe grandis (Nees) Merr. (Lauraceae). Compounds 2 and 3 were first time isolated as new naturally occurring compounds from plants. The NMR data for the compounds 2–4 have never been reported so far. Compounds 1 and 2 showed significant cytotoxic activity against a MCF7 (human estrogen receptor (ER+) positive breast cancer) cell line with IC50 values of 26 and 60 µg/mL, respectively. Furthermore, in vitro cytotoxic activity against HepG2 (human liver cancer) cell line was evaluated for compounds 1–4 with IC50 values of 27, 14, 81 and 20 µg/mL, respectively. Lysicamine (1) displayed strong antibacterial activity against Bacillus subtilis (B145), Staphylococcus aureus (S1434) and Staphylococus epidermidis (a clinically isolated strain) with inhibition zones of 15.50 ± 0.57, 13.33 ± 0.57 and 12.00 ± 0.00 mm, respectively. However, none of the tested pathogenic bacteria were susceptible towards compounds 2 and 3en-USPhoebe grandis; Lauraceae; aporphine alkaloid; cytotoxic; antibacterialArticle89949009188