Ong, EBBEBBOngAnthony, AAAAAnthonyIsmail, AAIsmailIsmail, AAIsmailLim, TSTSLim2024-05-292024-05-2920130732-889310.1016/j.diagmicrobio.2013.05.010WOS:000323467700018https://oarep.usim.edu.my/handle/123456789/12108The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using sera from typhoid patients showed the presence of HlyE-specific antibodies in circulation. (c) 2013 Elsevier Inc. All rights reserved.en-USSalmonellaTyphiHlyEAntigenImmunoblot, ELISAArticle8789771