Veeradasan PerumalUda HashimSubash C.B. GopinathR. HaarindraprasadP. PoopalanWei-Wen LiuM. RavichandranS.R. BalakrishnanA.R. Ruslinda2024-05-282024-05-282016Veeradasan Perumal, Uda Hashim, Subash C.B. Gopinath, R. Haarindraprasad, P. Poopalan, Wei-Wen Liu, M. Ravichandran, S.R. Balakrishnan, A.R. Ruslinda, A new nano-worm structure from gold-nanoparticle mediated random curving of zinc oxide nanorods, Biosensors and Bioelectronics, Volume 78, 2016, Pages 14-22, ISSN 0956-5663, https://doi.org/.10.1016/j.bios.2015.10.083https://www.sciencedirect.com/science/article/abs/pii/S0956566315305443https://oarep.usim.edu.my/handle/123456789/6977Biosensors and Bioelectronics Volume 78, 15 April 2016, Pages 14-22Creating novel nanostructures is a primary step for high-performance analytical sensing. Herein, a new worm like nanostructure with Zinc Oxide-gold (ZnO/Au) hybrid was fabricated through an aqueous hydrothermal method, by doping Au-nanoparticle (AuNP) on the growing ZnO lattice. During ZnO growth, fine tuning the solution temperature expedites random curving of ZnO nanorods and forms nano-worms. The nano-worms which were evidenced by morphological, physical and structural analyses, revealed elongated structures protruding from the surface (length: 1 µm; diameter: ~100 nm). The appropriate peaks for the face centred cubic gold were (111) and (200), as seen from X-ray diffractogram. The strong interrelation between Au and ZnO was manifested by X-ray photoelectron spectroscopy. The combined surface area increment from the nanoparticle radii and ZnO nanorod random curving gives raise an enhancement in detection sensitivity by increasing bio-loading. ‘Au-decorated hybrid nano-worm’ was immobilized with a probe DNA from Vibrio Cholera and duplexed with a target which was revealed by Fourier Transform Infrared Spectroscopy. Our novel Au-decorated hybrid nano-worm is suitable for high-performance bio-sensing, as evidenced by impedance spectroscopy, having higher-specificity and attained femtomolar (10 fM) sensitivity. Further, higher stability, reproducibility and regeneration on this sensing surface were demonstrated.enNano-worm, Nanorod, Zinc Oxide, Gold nanoparticle, DNA, CholeraArticle142278