Ameer Badr KhudhairTony HadibarataAbdull Rahim Mohd YusoffZee Chuang TehLiyana Amalina AdnanHesam Kamyab2024-05-282024-05-28201529/10/2019Khudhair, A.B., Hadibarata, T., Yusoff, A.R.M. et al. Pyrene Metabolism by New Species Isolated from Soil Rhizoctonia Zeae SOL3. Water Air Soil Pollut 226, 186 (2015). https://doi.org/10.1007/s11270-015-2432-40049-69792236-710.1007/s11270-015-2432-4https://link.springer.com/article/10.1007%2Fs11270-015-2432-4https://oarep.usim.edu.my/handle/123456789/4813Rhizoctonia zeae SOL3 fungus was isolated from contaminated soil based on its ability to decolorize remazol brilliant blue R in solid medium. This fungus has been used to degrade pyrene a four-ring polycyclic aromatic hydrocarbon. R. zeae SOL3 could biodegrade pyrene as a sole source of carbon and energy. Different parameters were investigated to study their effect on the biodegradation rate. The highest biodegradation rate reached at 28 °C, non-agitated culture, 20 g/L glucose, 24 g/L NaCl, and 20 mg/L pyrene. The metabolites of pyrene were detected by thin layer chromatography (TLC) and confirmed by gas chromatography–mass spectrometry (GC-MS), which were identified as benzoic acid, 4-hydroxybenzoic acid and botanic acid.enBiodegradationPolycyclic aromatic hydrocarbonPyrene metabolitesRhizoctonia zeae SOL3Article18602261