Publication: Internal Validation Of Reduced Pcr Reaction Volume Of The Qiagen Investigator® Argus X-12 Qs Kit From Blood Samples On Fta Cards
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Date
2023
Journal Title
Journal ISSN
Volume Title
Publisher
American Academy of Forensic Sciences.
Abstract
The onus of proof in criminal cases is beyond any reasonable doubt, and the issue on the lack of complete internal validation data can be manipulated when it comes to justifying the validity and reliability of the X-chromosomal short tandem repeats analysis for court representation. Therefore, this research evaluated the efficiency of the optimized 60% reduced volumes for polymerase chain reaction (PCR) amplification using the Qiagen Investigator® Argus X-12 QS Kit, as well as the capillary electrophoresis (CE) sample preparation for blood samples on Flinder's Technology Associates (FTA) cards. Good-quality DNA profile (3000–12,000 RFU) from the purified blood sample on FTA card (1.2 mm) were obtained using the optimized PCR (10.0 μL of PCR reaction volume and 21 cycles) and CE (9.0 μL Hi-Di™ Formamide and 0.3 μL DNA Size Standard 550 [BTO] and 27 s injection time) conditions. The analytical and stochastic thresholds were 100 and 200 RFU, respectively. Hence, the internal validation data supported the use of the optimized 60% reduced PCR amplification reaction volume of the Qiagen Investigator® Argus X-12 QS Kit as well as the CE sample preparation for producing reliable DNA profiles that comply with the quality assurance standards for forensic DNA testing laboratories, while optimizing the analytical cost.
Description
Journal of Forensic Sciences
Volume 68, Issue 6 (Page: 2103 - 2115)
Keywords
blood FTA card punches, forensic DNA analysis, internal validation, Qiagen Investigator® Argus X-12 QS Kit, reduced PCR volume, X-short tandem repeats
Citation
Aedrianee Reeza Alwi, Naji Arafat Mahat, Faezah Mohd Salleh, Seri Mirianti Ishar, Mohammad Rahim Kamaluddin, & Mohd. (2023). Internal validation of reduced PCR reaction volume of the Qiagen Investigator® Argus X‐12 QS Kit from blood samples on FTA cards. Journal of Forensic Sciences, 68(6), 2103–2115. https://doi.org/10.1111/1556-4029.15370
URI
https://onlinelibrary.wiley.com/doi/epdf/10.1111/1556-4029.15370
https://oarep.usim.edu.my/handle/123456789/19856
https://www.scopus.com/record/display.uri?eid=2-s2.0-85169454675&origin=resultslist&sort=plf-f&src=s&sid=c88e5e83a68935be482b0e1d02431a59&sot=b&sdt=b&s=TITLE-ABS-KEY%28Internal+validation+of+reduced+PCR+reaction+volume+of+the+Qiagen+Investigator%C2%AE+Argus+X-12+QS+Kit+from+blood+samples+on+FTA+cards%29&sl=143&sessionSearchId=c88e5e83a68935be482b0e1d02431a59&relpos=0
https://oarep.usim.edu.my/handle/123456789/19856
https://www.scopus.com/record/display.uri?eid=2-s2.0-85169454675&origin=resultslist&sort=plf-f&src=s&sid=c88e5e83a68935be482b0e1d02431a59&sot=b&sdt=b&s=TITLE-ABS-KEY%28Internal+validation+of+reduced+PCR+reaction+volume+of+the+Qiagen+Investigator%C2%AE+Argus+X-12+QS+Kit+from+blood+samples+on+FTA+cards%29&sl=143&sessionSearchId=c88e5e83a68935be482b0e1d02431a59&relpos=0