Publication: Internal Validation Of Reduced Pcr Reaction Volume Of The Qiagen Investigator® Argus X-12 Qs Kit From Blood Samples On Fta Cards
dc.contributor.author | Aedrianee Reeza Alwi | |
dc.contributor.author | Naji Arafat Mahat | |
dc.contributor.author | Faezah Mohd Salleh | |
dc.contributor.author | Seri Mirianti Ishar | |
dc.contributor.author | Mohammad Rahim Kamaluddin | |
dc.contributor.author | Mohd Radzniwan A. Rashid | |
dc.date.accessioned | 2024-06-16T06:34:32Z | |
dc.date.available | 2024-06-16T06:34:32Z | |
dc.date.issued | 2023 | |
dc.date.submitted | 2024-1-23 | |
dc.description | Journal of Forensic Sciences Volume 68, Issue 6 (Page: 2103 - 2115) | |
dc.description.abstract | The onus of proof in criminal cases is beyond any reasonable doubt, and the issue on the lack of complete internal validation data can be manipulated when it comes to justifying the validity and reliability of the X-chromosomal short tandem repeats analysis for court representation. Therefore, this research evaluated the efficiency of the optimized 60% reduced volumes for polymerase chain reaction (PCR) amplification using the Qiagen Investigator® Argus X-12 QS Kit, as well as the capillary electrophoresis (CE) sample preparation for blood samples on Flinder's Technology Associates (FTA) cards. Good-quality DNA profile (3000–12,000 RFU) from the purified blood sample on FTA card (1.2 mm) were obtained using the optimized PCR (10.0 μL of PCR reaction volume and 21 cycles) and CE (9.0 μL Hi-Di™ Formamide and 0.3 μL DNA Size Standard 550 [BTO] and 27 s injection time) conditions. The analytical and stochastic thresholds were 100 and 200 RFU, respectively. Hence, the internal validation data supported the use of the optimized 60% reduced PCR amplification reaction volume of the Qiagen Investigator® Argus X-12 QS Kit as well as the CE sample preparation for producing reliable DNA profiles that comply with the quality assurance standards for forensic DNA testing laboratories, while optimizing the analytical cost. | |
dc.identifier.citation | Aedrianee Reeza Alwi, Naji Arafat Mahat, Faezah Mohd Salleh, Seri Mirianti Ishar, Mohammad Rahim Kamaluddin, & Mohd. (2023). Internal validation of reduced PCR reaction volume of the Qiagen Investigator® Argus X‐12 QS Kit from blood samples on FTA cards. Journal of Forensic Sciences, 68(6), 2103–2115. https://doi.org/10.1111/1556-4029.15370 | |
dc.identifier.doi | 10.1111/1556-4029.15370 | |
dc.identifier.epage | 2115 | |
dc.identifier.issn | 0022-1198 | |
dc.identifier.issue | 68 | |
dc.identifier.spage | 2103 | |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/epdf/10.1111/1556-4029.15370 | |
dc.identifier.uri | https://oarep.usim.edu.my/handle/123456789/19856 | |
dc.identifier.uri | https://www.scopus.com/record/display.uri?eid=2-s2.0-85169454675&origin=resultslist&sort=plf-f&src=s&sid=c88e5e83a68935be482b0e1d02431a59&sot=b&sdt=b&s=TITLE-ABS-KEY%28Internal+validation+of+reduced+PCR+reaction+volume+of+the+Qiagen+Investigator%C2%AE+Argus+X-12+QS+Kit+from+blood+samples+on+FTA+cards%29&sl=143&sessionSearchId=c88e5e83a68935be482b0e1d02431a59&relpos=0 | |
dc.identifier.volume | 6 | |
dc.language.iso | en_US | |
dc.publisher | American Academy of Forensic Sciences. | |
dc.relation.ispartof | Journal of Forensic Sciences | |
dc.relation.issn | 1556-4029 | |
dc.relation.journal | Journal of Forensic Sciences | |
dc.subject | blood FTA card punches | |
dc.subject | forensic DNA analysis | |
dc.subject | internal validation | |
dc.subject | Qiagen Investigator® Argus X-12 QS Kit | |
dc.subject | reduced PCR volume | |
dc.subject | X-short tandem repeats | |
dc.title | Internal Validation Of Reduced Pcr Reaction Volume Of The Qiagen Investigator® Argus X-12 Qs Kit From Blood Samples On Fta Cards | |
dc.type | text::journal::journal article::research article | |
dspace.entity.type | Publication | |
oaire.citation.endPage | 2115 | |
oaire.citation.issue | 6 | |
oaire.citation.startPage | 2103 | |
oaire.citation.volume | 68 | |
oairecerif.author.affiliation | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
oairecerif.author.affiliation | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
oairecerif.author.affiliation | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
oairecerif.author.affiliation | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
oairecerif.author.affiliation | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
oairecerif.author.affiliation | Universiti Sains Islam Malaysia |