Publication:
Internal Validation Of Reduced Pcr Reaction Volume Of The Qiagen Investigator® Argus X-12 Qs Kit From Blood Samples On Fta Cards

dc.contributor.authorAedrianee Reeza Alwi
dc.contributor.authorNaji Arafat Mahat
dc.contributor.authorFaezah Mohd Salleh
dc.contributor.authorSeri Mirianti Ishar
dc.contributor.authorMohammad Rahim Kamaluddin
dc.contributor.authorMohd Radzniwan A. Rashid
dc.date.accessioned2024-06-16T06:34:32Z
dc.date.available2024-06-16T06:34:32Z
dc.date.issued2023
dc.date.submitted2024-1-23
dc.descriptionJournal of Forensic Sciences Volume 68, Issue 6 (Page: 2103 - 2115)
dc.description.abstractThe onus of proof in criminal cases is beyond any reasonable doubt, and the issue on the lack of complete internal validation data can be manipulated when it comes to justifying the validity and reliability of the X-chromosomal short tandem repeats analysis for court representation. Therefore, this research evaluated the efficiency of the optimized 60% reduced volumes for polymerase chain reaction (PCR) amplification using the Qiagen Investigator® Argus X-12 QS Kit, as well as the capillary electrophoresis (CE) sample preparation for blood samples on Flinder's Technology Associates (FTA) cards. Good-quality DNA profile (3000–12,000 RFU) from the purified blood sample on FTA card (1.2 mm) were obtained using the optimized PCR (10.0 μL of PCR reaction volume and 21 cycles) and CE (9.0 μL Hi-Di™ Formamide and 0.3 μL DNA Size Standard 550 [BTO] and 27 s injection time) conditions. The analytical and stochastic thresholds were 100 and 200 RFU, respectively. Hence, the internal validation data supported the use of the optimized 60% reduced PCR amplification reaction volume of the Qiagen Investigator® Argus X-12 QS Kit as well as the CE sample preparation for producing reliable DNA profiles that comply with the quality assurance standards for forensic DNA testing laboratories, while optimizing the analytical cost.
dc.identifier.citationAedrianee Reeza Alwi, Naji Arafat Mahat, Faezah Mohd Salleh, Seri Mirianti Ishar, Mohammad Rahim Kamaluddin, & Mohd. (2023). Internal validation of reduced PCR reaction volume of the Qiagen Investigator® Argus X‐12 QS Kit from blood samples on FTA cards. Journal of Forensic Sciences, 68(6), 2103–2115. https://doi.org/10.1111/1556-4029.15370
dc.identifier.doi10.1111/1556-4029.15370
dc.identifier.epage2115
dc.identifier.issn0022-1198
dc.identifier.issue68
dc.identifier.spage2103
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/epdf/10.1111/1556-4029.15370
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/19856
dc.identifier.urihttps://www.scopus.com/record/display.uri?eid=2-s2.0-85169454675&origin=resultslist&sort=plf-f&src=s&sid=c88e5e83a68935be482b0e1d02431a59&sot=b&sdt=b&s=TITLE-ABS-KEY%28Internal+validation+of+reduced+PCR+reaction+volume+of+the+Qiagen+Investigator%C2%AE+Argus+X-12+QS+Kit+from+blood+samples+on+FTA+cards%29&sl=143&sessionSearchId=c88e5e83a68935be482b0e1d02431a59&relpos=0
dc.identifier.volume6
dc.language.isoen_US
dc.publisherAmerican Academy of Forensic Sciences.
dc.relation.ispartofJournal of Forensic Sciences
dc.relation.issn1556-4029
dc.relation.journalJournal of Forensic Sciences
dc.subjectblood FTA card punches
dc.subjectforensic DNA analysis
dc.subjectinternal validation
dc.subjectQiagen Investigator® Argus X-12 QS Kit
dc.subjectreduced PCR volume
dc.subjectX-short tandem repeats
dc.titleInternal Validation Of Reduced Pcr Reaction Volume Of The Qiagen Investigator® Argus X-12 Qs Kit From Blood Samples On Fta Cards
dc.typetext::journal::journal article::research article
dspace.entity.typePublication
oaire.citation.endPage2115
oaire.citation.issue6
oaire.citation.startPage2103
oaire.citation.volume68
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliationUniversiti Sains Islam Malaysia

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