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Characterisation of cholinesterase from kidney tissue of Asian sea bass (Lates calcarifer) and its inhibition in presence of metal ions

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dc.contributor.authorHayat, NMen_US
dc.contributor.authorAhmad, SAen_US
dc.contributor.authorShamaan, NAen_US
dc.contributor.authorSabullah, MKen_US
dc.contributor.authorShukor, MYAen_US
dc.contributor.authorSyed, MAen_US
dc.contributor.authorKhalid, Aen_US
dc.contributor.authorKhalil, KAen_US
dc.contributor.authorDahalan, FAen_US
dc.date.accessioned2024-05-29T02:52:00Z
dc.date.available2024-05-29T02:52:00Z
dc.date.issued2017
dc.description.abstractAim : The cholinesterase (ChE) based inhibition studies from fish were investigated and presented here emerged to be one of the great potential biomarkers for heavy metals monitoring. Methodology : In this study, the capability of ChE extracted from the kidney of Lates calcarifer was assessed for of metal. ChE was purified through ammonium sulphate precipitation and ion exchange chromatography. Results : The purified enzyme gave 12 fold purification with the recovery of 12.17% with specific activity of 2.889 U mg(-1). The Michaelis-Menten constant (K-m) and V-max value obtained was 0.1426 mM and 0.0217 mu mol min(-1)mg(-1), respectively. The enzyme has the ability to hydrolyse acetylthiocholine iodide (ATC) at a faster rate compared to other two synthetic substrates, propionylthiocholine iodide (PTC) and butyrylthiocholine iodide (BTC). ChE gave highest activity at 20-30 degrees C in Tris-HCI buffer pH 8.0. The results showed that cholinesterase from L. calcarifer kidney was very sensitive to sensitive to copper and lead after being tested argentum, arsenic, cadmium, chromium, copper, cobalt, mercury, nickel, lead and zinc. Interpretation : The effect of heavy metals studied on the activity of ChE differed from each other. The result of the study can be used as a tool for further developing a biomarker for the detection of heavy metals in aquatic ecosystems. In addition, the information can also be used for designing a kit, that would give a rapid and accurate result.
dc.identifier.doi10.22438/jeb/38/3/MRN-987
dc.identifier.epage388
dc.identifier.issn0254-8704
dc.identifier.issue3
dc.identifier.scopusWOS:000400925700004
dc.identifier.spage383
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/11265
dc.identifier.volume38
dc.languageEnglish
dc.language.isoen_US
dc.publisherTriveni Enterprisesen_US
dc.relation.ispartofJournal Of Environmental Biology
dc.sourceWeb Of Science (ISI)
dc.subjectBiomarkeren_US
dc.subjectCholinesteraseen_US
dc.subjectMetal ionsen_US
dc.subjectLates calcariferen_US
dc.titleCharacterisation of cholinesterase from kidney tissue of Asian sea bass (Lates calcarifer) and its inhibition in presence of metal ions
dc.typeArticleen_US
dspace.entity.typePublication

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