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Phytochemical analysis of Quercus infectoria galls extracts using FTIR, LC-MS and MS/MS analysis

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dc.contributor.authorAbdullah, AR;en_US
dc.contributor.authorHapidin, H;en_US
dc.contributor.authorAbdullah, Hen_US
dc.date.accessioned2024-05-27T14:41:53Z
dc.date.available2024-05-27T14:41:53Z
dc.date.issued2017
dc.description.abstractThe present study was carried out to characterise and compare types of phytoconstituents present in crude and aqueous extract of Quercus infectoria (QI) using FTIR, LC-MS followed by confirming using MS/MS analysis. QI gall was purchased from local market and grinded to obtain the crude in powdered form. The aqueous extract produced by refluxing in water bath at 50°C for 24 hours was filtered and concentrated using rotary evaporator followed by freeze-drying. The crude and aqueous extract was scanned at wavelength 400 to 3400 nm using Tensor-II-FTIR spectrometer. LC-MS analysis utilised Agilent-1290-Infinity-system coupled to Agilent-6520-Accurate-Mass-Q-TOF mass-spectrometer with dual ESI. Detected constituents matched with METLIN_AM_PCDL-N-130328.cdb database. Compounds with >95 DB score were selected to be confirmed with MS/MS analysis. The FTIR spectrum confirmed presence of several similar functional groups in both crude and aqueous extract. LC-MS screening results indicated similar phytoconstituents present in both crude and aqueous QI (2.64% and 2.16%) respectively. MS/MS analysis confirmed presence of all targeted constituents in aqueous QI extract. In conclusion, LC-MS and MS/MS results show that all major constituents analysed were from polyphenolic derivatives primarily gallic acid and hydrolysable tannins.en_US
dc.identifier.citationRESEARCH JOURNAL OF BIOTECHNOLOGY, 2017; 12 (12): 55en_US
dc.identifier.epage61
dc.identifier.issn0973-6263
dc.identifier.issue12
dc.identifier.other2348-3
dc.identifier.spage55
dc.identifier.urihttps://www.medsci.cn/sci/show_paper.asp?id=885ee11ac1232547
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/3625
dc.identifier.volume12
dc.language.isoenen_US
dc.publisherResearch Journal of BioTechnologyen_US
dc.relation.ispartofResearch Journal Of Biotechnologyen_US
dc.subjectFTIR,en_US
dc.subjectLC-MS,en_US
dc.subjectMS/MS,en_US
dc.subjectPhytochemical,en_US
dc.subjectQuercus infectoria (QI).en_US
dc.titlePhytochemical analysis of Quercus infectoria galls extracts using FTIR, LC-MS and MS/MS analysisen_US
dc.typeArticleen_US
dspace.entity.typePublication

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