Publication: Isolation and Production of Polysaccharide from Locally Isolated Termitomyces sp. Mushroom
Loading...
Date
2020-12-25
Journal Title
Journal ISSN
Volume Title
Publisher
Penerbit USIM, Universiti Sains Islam Malaysia
Abstract
Termitomyces sp. mushroom is an edible mushroom that belongs to the family of Lyophyllaceae and exhibited growth associated with termites in a symbiotic environment. In this study, Termitomyces sp. mushroom was isolated from several places located in Negeri Sembilan, Malaysia namely TM 1 (Senaling, Kuala Pilah, TM 2 (Batu Kikir, Kuala Pilah) and TM 3 (Rembau Negeri Sembilan). The collection process was conducted during the early rainy seasons within October to November in which these mushrooms were actively growing. The collected mushrooms fruiting bodies from the wild were further used in tissue culture for the growth of mycelium on the agar medium. Out of the three samples only TM 1 mycelium were successfully grown on the agar medium and subsequent optimization were carried out to enhance the growth of the TM 1 mycelium. The composition of the agar medium was manipulated, and it was observed that the combination of PDA (potato dextrose agar) + ME (malt extract) + YE (yeast extract) showed a thick full plate growth of mycelium in 14 days of cultivation. The mushroom mycelium of TM 1 was able to grow and produce polysaccharide in batch submerged liquid fermentation with a biomass of 8.55 g/L, intracellular polysaccharide (IPS CWE and IPS HWE) of 1.36 and 3.20 g/L respectively and extracellular polysaccharide of 1.44 g/L.
Description
E-Proceeding Kolokium Siswazah Fakulti Sains Dan Teknologi 2020 (KOSIST 2020)
“Synergizing Innovation and Research Through Science and Technology”
9th December 2020
Organized by Faculty Science and Technology
Keywords
Termitomyces; mushroom; polysaccharide; submerged fermentation