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Isolation and Production of Polysaccharide from Locally Isolated Termitomyces sp. Mushroom

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dc.contributor.authorSrivani Sellathoroeen_US
dc.contributor.authorTengku Shafazila Tengku Saharuddinen_US
dc.contributor.authorEhwan Ngadien_US
dc.contributor.authorWan Abd Al Qadr Imad Wan-Mohtaren_US
dc.contributor.authorIffah Izzati Zakariaen_US
dc.contributor.authorRahayu Ahmaden_US
dc.date.accessioned2024-05-30T07:49:02Z
dc.date.available2024-05-30T07:49:02Z
dc.date.issued2020-12-25
dc.descriptionE-Proceeding Kolokium Siswazah Fakulti Sains Dan Teknologi 2020 (KOSIST 2020) “Synergizing Innovation and Research Through Science and Technology” 9th December 2020 Organized by Faculty Science and Technologyen_US
dc.description.abstractTermitomyces sp. mushroom is an edible mushroom that belongs to the family of Lyophyllaceae and exhibited growth associated with termites in a symbiotic environment. In this study, Termitomyces sp. mushroom was isolated from several places located in Negeri Sembilan, Malaysia namely TM 1 (Senaling, Kuala Pilah, TM 2 (Batu Kikir, Kuala Pilah) and TM 3 (Rembau Negeri Sembilan). The collection process was conducted during the early rainy seasons within October to November in which these mushrooms were actively growing. The collected mushrooms fruiting bodies from the wild were further used in tissue culture for the growth of mycelium on the agar medium. Out of the three samples only TM 1 mycelium were successfully grown on the agar medium and subsequent optimization were carried out to enhance the growth of the TM 1 mycelium. The composition of the agar medium was manipulated, and it was observed that the combination of PDA (potato dextrose agar) + ME (malt extract) + YE (yeast extract) showed a thick full plate growth of mycelium in 14 days of cultivation. The mushroom mycelium of TM 1 was able to grow and produce polysaccharide in batch submerged liquid fermentation with a biomass of 8.55 g/L, intracellular polysaccharide (IPS CWE and IPS HWE) of 1.36 and 3.20 g/L respectively and extracellular polysaccharide of 1.44 g/L.en_US
dc.identifier.epage40
dc.identifier.isbne-ISBN: 978-967-440-873-2
dc.identifier.spage36
dc.identifier.urihttps://kosist2020.wixsite.com/website/e-proceeding
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/17825
dc.language.isoen_USen_US
dc.publisherPenerbit USIM, Universiti Sains Islam Malaysiaen_US
dc.relation.conferenceE-Proceeding Kolokium Siswazah Fakulti Sains Dan Teknologi 2020 (KOSIST 2020)en_US
dc.subjectTermitomyces; mushroom; polysaccharide; submerged fermentationen_US
dc.titleIsolation and Production of Polysaccharide from Locally Isolated Termitomyces sp. Mushroomen_US
dc.typeArticleen_US
dspace.entity.typePublication

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