Publication:
Inhibition Mechanism Of Silver Nanoparticle-kaempferol Against Methicillin-resistant Staphylococcus Aureus

Simple item page
dc.contributor.authorNur Farah Atiqah Mohd Pazli
dc.contributor.authorSiti Aisyah Abd Ghafar
dc.contributor.authorAriff Haikal Hairil Anuar
dc.contributor.authorRohazila Mohamad Hanafiah
dc.date.accessioned2024-07-13T15:15:40Z
dc.date.available2024-07-13T15:15:40Z
dc.date.issued2024
dc.date.submitted2024-7-10
dc.descriptionArabian Journal of Chemistry, Volume 17 Issue 2
dc.description.abstractMethicillin-resistant Staphylococcus aureus (MRSA), a multidrug resistant strain, is known to cause a threat to public health due to its limited therapeutic treatment. Kaempferol (K) is a natural flavonoid that shows antibacterial activities toward MRSA, but its effectiveness is limited due to its low water solubility. However, poorly aqueous soluble drugs displayed better solubility through nano formulation. Hence, kaempferols were incorporated with silver nanoparticles (AgNPs) to enhance their solubility and antibacterial activity. Previous study showed that AgNPs incorporated with kaempferol (AgNPs-K) exhibited antibacterial activity against MRSA. However, the knowledge regarding the mechanism of action AgNPs-K against MRSA is still limited. The objective of the study is to unravel the inhibition mechanism of silver nanoparticles-kaempferol (AgNPs-K) on treated MRSA. The scanning electron microscopy (SEM) result showed significant difference in morphology between treated and non-treated MRSA which suggest the effectiveness of the AgNPs-K. Non-treated MRSA has an oval shape while MRSA treated with AgNPs-K showed a disrupted cell wall with contents leakage. The transcriptomic profile analysis by Next Generation Sequencing (NGS) showed that various genes and pathways related to biofilm, virulent activity and glycolysis pathway are differently expressed, with 581 genes were downregulated and 641 were upregulated. The affected genes of icab, clfa and eno which involved in biofilm, clumping factor A (virulent) and glycolysis pathway were validated by RT-PCR technique. The results were consistent with the NGS outcome. In conclusion, AgNPs-K possesses antibacterial activity against MRSA and its mechanism of action are reflected in the gene expression of biofilm pathway, virulent and glycolysis activity. Therefore, AgNPs-K can be suggested as a potential alternative to combat MRSA infection.
dc.identifier.citationNur Farah Atiqah Mohd Pazli, Siti Aisyah Abd Ghafar, Ariff Haikal Hairil Anuar, Rohazila Mohamad Hanafiah (2024). Inhibition mechanism of silver nanoparticle-kaempferol against methicillin-resistant Staphylococcus aureus. Arabian Journal of Chemistry, 17(2), 105489. https://doi.org/10.1016/j.arabjc.2023.105489
dc.identifier.doi10.1016/j.arabjc.2023.105489
dc.identifier.epage105493
dc.identifier.issn1878-5352
dc.identifier.issue2
dc.identifier.other1209-27
dc.identifier.spage105489
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/20647
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1878535223009516?via%3Dihub
dc.identifier.volume17
dc.language.isoen_US
dc.publisherElsevier
dc.relation.ispartofArabian Journal of Chemistry
dc.relation.issn1878-5352
dc.relation.journalArabian Journal of Chemistry
dc.subjectSilver nanoparticles Kaempferol Methicillin-resistant Staphylococcus aureus Transcriptomic profile analysis
dc.titleInhibition Mechanism Of Silver Nanoparticle-kaempferol Against Methicillin-resistant Staphylococcus Aureus
dc.typetext::journal::journal article::research article
dspace.entity.typePublication
oaire.citation.endPage11
oaire.citation.issue2
oaire.citation.startPage1
oaire.citation.volume17
oairecerif.author.affiliationUniversiti Sains Islam Malaysia
oairecerif.author.affiliationUniversiti Sains Islam Malaysia
oairecerif.author.affiliationUniversiti Sains Islam Malaysia
oairecerif.author.affiliationUniversiti Sains Islam Malaysia

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Inhibition mechanism of silver nanoparticle-kaempferol against methicillin-resistant Staphylococcus aureus
Size:
4.63 MB
Format:
Adobe Portable Document Format
Download

Collections

Scopus