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Widefield Two-photon Excitation Without Scanning: Live Cell Microscopy With Hightime Resolution And Low Photo-bleaching

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dc.contributor.authorNor Zaihana Abdul Rahmanen_US
dc.contributor.authorRumelo Amoren_US
dc.contributor.authorAlison McDonalden_US
dc.contributor.authorJohanna Trägårdhen_US
dc.contributor.authorGillian Robben_US
dc.contributor.authorLouise Wilsonen_US
dc.contributor.authorJohn Dempsteren_US
dc.contributor.authorWilliam Bradshaw Amos1en_US
dc.contributor.authorTrevor J. Bushellen_US
dc.contributor.authorGail McConnellen_US
dc.date.accessioned2024-05-28T04:32:41Z
dc.date.available2024-05-28T04:32:41Z
dc.date.issued2016
dc.description.abstractWe demonstrate fluorescence imaging by two-photon excitation without scanning in biological specimens as previously described by Hwang and co-workers, but with an increased field size and with framing rates of up to 100 Hz. During recordings of synaptically-driven Ca2+ events in primary rat hippocampal neurone cultures loaded with the fluorescent Ca2+ indicator Fluo-4 AM, we have observed greatly reduced photo-bleaching in comparison with single-photon excitation. This method, which requires no costly additions to the microscope, promises to be useful for work where high time-resolution is required.en_US
dc.identifier.doi10.1371/journal.pone.0147115
dc.identifier.epage19
dc.identifier.issn1932-6203
dc.identifier.spage1
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/5846
dc.language.isoen_USen_US
dc.publisherAmor et alen_US
dc.relation.ispartofPlos One Journalen_US
dc.subjectFluorescence imaging, Lasers, Optical lenses, Specimen sectioning,Two-photon excitation microscopy, Fluorescence, Fluorescence microscopy, Imaging techniquesen_US
dc.titleWidefield Two-photon Excitation Without Scanning: Live Cell Microscopy With Hightime Resolution And Low Photo-bleachingen_US
dc.typeArticleen_US
dspace.entity.typePublication

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