Publication: Simple Colorimetric DNA Biosensor Based on Gold Nanoparticles for Pork Adulteration Detection in Processed Meats
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Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
International Frequency Sensor Association (IFSA)
Abstract
In order to develop simple sensing system for pork adulteration in processed meats, the DNA biosensor based on 20 nm gold nanoparticles (AuNPs) with aggregation property has been developed as a simple colorimetric detection of target DNA. Adsorption of single-stranded (ss) DNA on AuNPs protects the particles against salt-induced aggregation. However, mixing and annealing of a 27-nucleotide (nt) ssDNA probe on AuNPs with denatured DNA of different processed meats differentiated between perfectly matched and mismatch hybridization at a critical annealing temperature (55 °C). The AuNPs change color from red to purple, in 10 mM phosphate buffer saline (PBS). At a hybridizing temperature (55 °C), non-target mismatched DNA provided hybridization products allowing probe to be free and adsorbed to AuNPs. This prevented AuNPs from salt-induced aggregation as the color still red. In matched DNA, hybridization would occurred, allowing probe to be occupied. This facilitated AuNPs from salt-induced aggregation and induced colorimetric change of particles from red to purple. These signals could be observed easily with naked eye. This label-free DNA nanobiosensor should find applications in food analysis and other DNA based screening.
Description
Keywords
Colorimetric sensors,, DNA biosensor,, Gold nanoparticles,, Pork adulteration,, Processed meats
Citation
Sensors & Transducers, Vol. 208, Issue 1, January 2017, pp. 7-13