Publication:
Simple Colorimetric DNA Biosensor Based on Gold Nanoparticles for Pork Adulteration Detection in Processed Meats

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dc.contributor.authorBambang Kuswandien_US
dc.contributor.authorAgus A Ganien_US
dc.contributor.authorNia Kristiningrumen_US
dc.contributor.authorMusa Bin Ahmaden_US
dc.date.accessioned2024-05-28T04:21:11Z
dc.date.available2024-05-28T04:21:11Z
dc.date.issued2017
dc.description.abstractIn order to develop simple sensing system for pork adulteration in processed meats, the DNA biosensor based on 20 nm gold nanoparticles (AuNPs) with aggregation property has been developed as a simple colorimetric detection of target DNA. Adsorption of single-stranded (ss) DNA on AuNPs protects the particles against salt-induced aggregation. However, mixing and annealing of a 27-nucleotide (nt) ssDNA probe on AuNPs with denatured DNA of different processed meats differentiated between perfectly matched and mismatch hybridization at a critical annealing temperature (55 °C). The AuNPs change color from red to purple, in 10 mM phosphate buffer saline (PBS). At a hybridizing temperature (55 °C), non-target mismatched DNA provided hybridization products allowing probe to be free and adsorbed to AuNPs. This prevented AuNPs from salt-induced aggregation as the color still red. In matched DNA, hybridization would occurred, allowing probe to be occupied. This facilitated AuNPs from salt-induced aggregation and induced colorimetric change of particles from red to purple. These signals could be observed easily with naked eye. This label-free DNA nanobiosensor should find applications in food analysis and other DNA based screening.en_US
dc.identifier.citationSensors & Transducers, Vol. 208, Issue 1, January 2017, pp. 7-13en_US
dc.identifier.epage13
dc.identifier.issn1726-5479
dc.identifier.issue1
dc.identifier.other1450-28
dc.identifier.spage7
dc.identifier.urihttps://www.proquest.com/docview/1866018954?pq-origsite=gscholar&fromopenview=true
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/5283
dc.identifier.volume208
dc.language.isoenen_US
dc.publisherInternational Frequency Sensor Association (IFSA)en_US
dc.relation.ispartofSensors & Transducersen_US
dc.subjectColorimetric sensors,en_US
dc.subjectDNA biosensor,en_US
dc.subjectGold nanoparticles,en_US
dc.subjectPork adulteration,en_US
dc.subjectProcessed meatsen_US
dc.titleSimple Colorimetric DNA Biosensor Based on Gold Nanoparticles for Pork Adulteration Detection in Processed Meatsen_US
dc.typeArticleen_US
dspace.entity.typePublication

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