Publication:
Critical Evaluation of Green Synthesized Silver Nanoparticles-Kaempferol for Antibacterial Activity Against Methicillin-Resistant Staphylococcus aureus

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dc.contributor.authorAriff Haikal Hairil Anuar
dc.contributor.authorSiti Aisyah Abd Ghafar
dc.contributor.authorRohazila Mohamad Hanafiah
dc.contributor.authorVuanghao Lim
dc.contributor.authorNur Farah Atiqah Mohd Pazli
dc.date.accessioned2024-07-15T17:04:23Z
dc.date.available2024-07-15T17:04:23Z
dc.date.issued2024
dc.date.submitted2024-7-10
dc.descriptionInternational Journal of Nanomedicine Volume 19 (page: 1339-1350)
dc.description.abstractIntroduction: This study aimed to characterize silver nanoparticles-kaempferol (AgNP-K) and its antibacterial activities against methicillin-resistant Staphylococcus aureus (MRSA). Green synthesis method was used to synthesize AgNP-K under the influence of temperature and different ratios of silver nitrate (AgNO3 and kaempferol). Methods: AgNP-K 1:1 was synthesized with 1 mM kaempferol, whereas AgNP-K 1:2 with 2 mM kaempferol. The characterization of AgNP-K 1:1 and AgNP-K 1:2 was performed using UV–visible spectroscopy (UV–Vis), Zetasizer, transmission electron microscopy (TEM), scanning electron microscopy-dispersive X-ray spectrometer (SEM-EDX), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy. The antibacterial activities of five samples (AgNP-K 1:1, AgNP-K 1:2, commercial AgNPs, kaempferol, and vancomycin) at different concentrations (1.25, 2.5, 5, and 10 mg/mL) against MRSA were determined via disc diffusion assay (DDA), minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) assay, and time-kill assay. Results: The presence of a dark brown colour in the solution indicated the formation of AgNP-K. The UV–visible absorption spectrum of the synthesized AgNP-K exhibited a broad peak at 447 nm. TEM, Zetasizer, and SEM-EDX results showed that the morphology and size of AgNP-K were nearly spherical in shape with 16.963 ± 6.0465 nm in size. XRD analysis confirmed that AgNP-K had a crystalline phase structure, while FTIR showed the absence of (-OH) group, indicating that kaempferol was successfully incorporated with silver. In DDA analysis, AgNP-K showed the largest inhibition zone (16.67 ± 1.19 mm) against MRSA as compared to kaempferol and commercial AgNPs. The MIC and MBC values for AgNP-K against MRSA were 1.25 and 2.50 mg/mL, respectively. The time-kill assay results showed that AgNP-K displayed bacteriostatic activity against MRSA. AgNP-K exhibited better antibacterial activity against MRSA when compared to commercial AgNPs or kaempferol alone.
dc.identifier.doi10.2147/IJN.S431499
dc.identifier.epage1350
dc.identifier.issn1178-2013
dc.identifier.issue19
dc.identifier.spage1339
dc.identifier.urihttps://www.dovepress.com/critical-evaluation-of-green-synthesized-silver-nanoparticles-kaempfer-peer-reviewed-fulltext-article-IJN
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/21916
dc.identifier.volume2024
dc.language.isoen_US
dc.publisherTaylor & Francis
dc.relation.ispartofInternational Journal of Nanomedicine
dc.relation.issn1339-1350
dc.relation.journalInternational Journal of Nanomedicine
dc.subjectnanoparticles
dc.subjectsilver nanoparticles
dc.subjectgreen synthesis
dc.subjectkaempferol
dc.subjectmethicillin-resistant Staphylococcus aureus
dc.titleCritical Evaluation of Green Synthesized Silver Nanoparticles-Kaempferol for Antibacterial Activity Against Methicillin-Resistant Staphylococcus aureus
dc.typetext::journal::journal article::research article
dspace.entity.typePublication
oaire.citation.endPage1350
oaire.citation.startPage1339
oaire.citation.volume19
oairecerif.author.affiliationUniversiti Sains Islam Malaysia
oairecerif.author.affiliationUniversiti Sains Islam Malaysia
oairecerif.author.affiliationUniversiti Sains Islam Malaysia
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliationUniversiti Sains Islam Malaysia

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