Publication: Antifungal Activity of Lactic Acid Bacteria Isolated from Soil Rhizosphere on Fusarium Species Infected Chilli Seeds
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Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Global Society of Scientific Research and Researchers
Abstract
Lactic acid bacteria (LAB) are known to inhibit the growth of fungi and LAB cells or its can be used as biocontrol against plant pathogens. The aim of this study was to evaluate the efficacy of selected LAB strains isolated from rhizospheric soil against Fusarium spp. These fungi were isolated from infected different plant parts and identified as F. oxysporum strain KAML01, Fusarium sp. CID124, Fusarium sp. fus 124 and F. proliferatum with 99 % similarity using Internal Transcribed Spacer (ITS) analysis. Five LAB strains were showed strong antifungal activity against the Fusarium spp. were evaluated using dual overlay method. Isolates MSS1 and FCF11 were 100% similarity with Lactobacillus plantarum and isolate MSS5 99% was similarity with Pediococcus pentoceous using 16S rDNA sequence analysis. Inhibition of Fusarium sp. CID124 fungal biomass in the de Man, Rogosa and Sharpe Broth (MRSB) was significantly greater (P ≤ 0.05) by cells of P. pentosaceus (97.43%) compared to inhibition of Fusarium sp. CID124 fungal biomass by Cell Free Supernatant (CFS) of Lb.
Description
Keywords
Antifungal activity; chilli seeds germination; Fusarium spp; Lactic Acid Bacteria; Rhizospheric soil.