Identification of Bacteria Sample Plaque from Periodontal Disease Among Diabetic Patients

Abstract

Molecular identiβication of bacteria are very important to develop databasegene bank in university. Databases of genes are very important to anno-tate a collection of nucleotide sequence and protein translation of oral bac-teria. This research was conducted to identify bacteria that present in theplaque sample among diabetic patients collected from the Department ofPeriodontology and Community Oral health Universiti Sains Islam Malaysia(USIM). A total of bacteria was successfully isolated on Blood Agar from peri-odontal patients’ plaques. Colony Forming Unit (CFU/mL) was calculated toestimate the number of viable bacteria in a sample after performed serialdilution. Gram stained was done for all isolated samples to differentiatebetween Gram positive and negative organisms. About 2.07 x 107CFU/mLof bacteria was successfully collected from periodontal plaques on diabetespatient. From the numbers, only 17 were selected to identify the species.Seven from the samples were Gram-positive bacteria, meanwhile ten sam-ples were identiβied as Gram-negative bacteria. PCR products were isolatedfrom the samples by using polymerase chain reaction analysis.Streptococ-cus mutans,Streptococcus gordonii,Streptococcus oralis,Streptococcus sangui-nis,Streptococcus agalactiae,Streptococcus lutetiensis,Streptococcus downei,,Aggregatibacter actinomycetemcomitans, Klebsiella pneumoniae,Enterobacterclocae,Salmonella enterica subs enterica,Enterobacter ludwigii, Enterobactermori,Enterobacter roggen kampii,Enterobacter cloacae,Enterobacter tabaciandKlebsiella pneumoniaewere identiβied from all samples via DNA sequenc-ing.