Black glutinous rice (Mhor37) is one of the plants that contain anthocyanin. Which is a naturally occurring pigment and will be able to change color according to the pH level. The efficacy of black glutinous rice (Mhor37) extract was studied by using a solvent of acetic acid and ethanol at concentrations of 30%, 45%, and 60% with a ratio of 1:1 (g/ml) for 12 hr and 24 hr. After that, take the extracts extracted with acetic acid at a concentration of 30% for 12 hr adjusted the pH level to 1.5, 3.0, 4.5, 6.0, and 7.5. The experimental results show that the extract from black glutinous rice (Mhor37) extracted with 30% acetic acid at pH 1.5 was efficient in chromosome staining at onion roots and it can clearly observe each phase of cell division. This project is expected to reduce the use of chemicals in chromosome staining, it may result in less chemical contamination in the environment and it also reduces the budget for the purchase of expensive chemical dyes.